Allergy.  1995 Jun;15(2):250-261.

Immunological Responres to Choronomus flaviplumus in Atopic Children

• Kim DS
• Lee KY
• Kang HY
• Kim KE
• Jeong BJ
• Ree HI

Abstract

Hypersensitivity to chironomidas(non-biting midge) has been commonly found in many countries. To evaluate the role of chirono~nidae as an inhalant allergen in childhood arcpie patients, we performed allergy skin test to crude extracts of Chironoraidae flaviplumus(CF) in 456 patients. Positive skin reactions were noted in 35 out of 456(7.8%) arcpie patients. All of the positive reactors also showed positive responses to other inhalant allergens, especially Dermatophagoides farinae. Specific IgE to CF antigens by ELISA were detected in 3 out of 8 patients and specific IgG and IgG subclasses were noted in 8 patients with very low absorbance value. SDS-PAGE analysis of CF extracts contains at least 16 antigenie components and 15 IgE binding components were noted by immunoblotting analysis. Among these allergenic corn ponents, three components of 32,000, 27,000, 19,000 dalton are bound strongly with the sera of arcpie patients tested. In conclusion, there was a low sensitization rates of CF antigen and low detection rate of CF-specific IgE and IgG. Further studies are needed to investigate its importance as an inhalant allergen in childhood patients and the pathogenic role of specific IgE and IgG antibodies to CF antigens.

MeSH Terms

Allergens
Antibodies
Child*
Complex Mixtures
Dermatophagoides farinae
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Hypersensitivity
Immunoblotting
Immunoglobulin E
Immunoglobulin G
Skin
Skin Tests
Zea mays
Allergens
Antibodies
Complex Mixtures
Immunoglobulin E
Immunoglobulin G