Tissue Eng Regen Med.  2016 Dec;13(6):724-731. 10.1007/s13770-016-0009-y.

The Expression Profile of Angiotensin System on Thawed Murine Ovaries

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. jyhsyk@snu.ac.kr
  • 2Department of Obstetrics and Gynecology, Korea University Guro Hospital, Seoul, Korea.
  • 3Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
  • 4Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
  • 55Department of Molecular Science, Ajou University, Suwon, Korea.

Abstract

Freezing and thawing is one of the most widely used tissue engineering techniques for the preservation of ovaries. Many cells and tissues demonstrate changes in functional gene expression after thawing. Several studies have reported the important roles of angiotensin (AT) system during the ovarian follicular growth. AT system consists of ATII, and ATII receptors type I (ATII-RI) and type II (ATII-RII). However, little is known whether frozen-thawed ovaries show any alteration of AT system member gene expression when treated with survival-enhancing factors. We aimed to investigate whether mass freezing and thawing with or without the use of Rho-associated kinase (ROCK) inhibitors up- or down-regulate the expression of ATII, ATII-RI, and ATII-RII genes on frozen-thawed ovarian tissues. Significant changes in the expression of ATII, ATII-RI, and ATII-RII genes were observed on thawed ovaries when compared to fresh control. The treatment with ROCK inhibitors did not significantly alter their expression. In conclusion, freezing and thawing of ovarian tissue may affect the mRNA expression levels of intra-ovarian AT system genes, and modulation of ROCK inhibitor activity may not regulate AT system on the frozenthawed ovarian tissue.

Keyword

Angiotensin; Freezingv; Ovary; Rho-associated kinase; Thawing

MeSH Terms

Angiotensins*
Female
Freezing
Gene Expression
Ovary*
rho-Associated Kinases
RNA, Messenger
Tissue Engineering
Angiotensins
RNA, Messenger
rho-Associated Kinases
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