J Korean Ophthalmol Soc.  2008 Nov;49(11):1839-1844.

The Effect of Sympathectomy on Circardian Rhythm of Intraocular Pressure in Rat

• Lim NK
• Kim TH
• Song JK
• Yoo JM

Abstract

PURPOSE
The present study was undertaken to determine the 24 - hour pattern of intraocular pressure (IOP), and to reveal the effect of unilateral decentralization on circardian rhythm of IOP in eyes of rats.
METHODS
Nine male Sprague - Dawley rats were exposed to a 12 - hour light / dark cycle for 3 weeks. 24-hours IOP pattern was measured at six time points (8, 12, 16, 20, 24, and 4 circardian time) by Tonopen XL tonometer in awake state. After unilateral sympathectomy (cervical ganglionectomy), IOP measurements were performed at the same time points on post-operation 1, 2, 3, and 4 weeks.
RESULTS
24 - hour IOP pattern of rats showed a rhythmic pattern that appeared to be sinusoidal with 12 - hour light / dark cycle. IOPs were low during the light phase and high during dark phase. After unilateral sympathectomy, the circardian rhythm of IOP was maintained in control eyes. But in sympathecotmized eyes, the circardian thythm of IOP was loss.
CONCLUSIONS
These results show that sympathetic nervous systems contribute to the circardian rhythm of IOP in rats.

Keyword

Circardian rhythm; Intraocular pressure; Sympathectomy

MeSH Terms

Animals
Eye
Humans
Intraocular Pressure
Light
Male
Politics
Rats
Sympathectomy
Sympathetic Nervous System

Figure

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  Figure 1. Twenty-four-hour IOP pattern of SD rats exposed to 12-hour light/dark cycle. Circardian rhythm of IOP showed a biphasic pattern that appeared to be sinusoidal with 12-hour light/dark cycle. In the dark period, more elevated IOPs were checked than in the light period ( P<0.01). † indicates that IOP of rats in the dark period was higher than in the light period.

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  Figure 2. Change of 24-hours IOP pattern of rats after unilateral cervicalganglionectomy. After unilateral sympathectomy, the circardian rhythm of IOP in unoperated side was maintained as similar pattern in pre-sympathectomy. But in operated side, the circardian thythm of IOP was disappeared. At 1 and 2 weeks, there was a difference of IOP in both eyes in the light & dark period. But after 3 weeks, the difference of IOP in both eyes can be only seen in the dark period ( P<0.01).* indicate the points that present difference of IOP in both eyes; OD=eye of operated side; OS=eye of unoperated side.

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  Figure 3. Unilateral cervical ganglionectomy was done in the right side of the rats. One day after operation, generalized ptosis appeared on the operated side (A. preoperative phtograph, B. intraoperative photograph, C. postoperative photograph).

Reference

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