J Korean Cancer Assoc.
1998 Jun;30(3):508-520.
Effect on Malignant Phenotype of Gastric Cancer Cell Line after p53 Gene Transduction
- Affiliations
-
- 1Yonsei Cancer Research Institute, Yonsei University College of Medicine, Seoul, Korea.
- 2Yonsei Cancer Center, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
- 4Department of General Surgery, Yonsei University College of Medicine, Seoul, Korea.
- 5Department of Internal Medicine, Ajou University School of Medicine, Suweon, Korea.
Abstract
- PURPOSE
To evaluate the effect of wild type p53 gene transduction on the malignant phenotypes for metastasis in gastric cancer, we compared the biological phenpotypes of gastric cancer cell lines based on p53 gene status. Then, after retrovirus-mediated wild-type p53 gene transduction, we compared those phenotypes among parent YCC-3 cell line, vector transduced YCC-3v cell line and a clone of YCC-3C3. MATERIAL AND METHODS: Four human gastric cancer celi lines were used; YCC-l(mutant), YCC-2(wild), YCC-3(mutant) and AGS(wild). DNAs of the cell lines were analyzed to evaluate the mobility shift with PCR-SSCP. Tumorigenecity and proliferation were evaluated by soft agar assay and proliferation assay. Migratory capacity was measured by adhesion assay and Boyden chamber assay. p53 protein expression was measured by Western blot analysis and VEGF, WAF-1 were measured by ELISA assay. Angiogenic activity was measured by cross-feeding assay and cell cycle analysis was performed by flowcytometry. In vivo tumorigenicity was measured by xenograft in nude mice.
RESULTS
YCC-3 cell line with mutant p53 gene expressed all the phenotypes for the metastasis such as tumorigenicity, migration and angiogenesis. In a stable clone of YCC-3C3, no differences were found in proliferation, cell cycle and WAP-1 expression when compared to those of the control YCC-3v and parent YCC-3 cell line, even if increased p53 protein production was found by Western blot analysis. However, both in vitro and in vivo tumorigenicity were decreased in a stably transduced YCC-3C3 clone. The adhesive capacity was also decreased in YCC-3C3 clone whereas the endothelial cell growth stimulatory effect and VEGF production showed no difference compared to those of the YCC-3v cell line.
CONCLUSION
Wild-type p53 gene transduction in gastric cancer cell line decreased tumorigenicity which resulted from decreased colony forming activity and adhesive capacity but not formed changes of angiogenic activity. This suggested the possible application of anti- metastasis strategy with p53 gene therapy in gastric cancer.
MeSH Terms
-
Adhesives
Agar
Animals
Blotting, Western
Cell Cycle
Cell Line*
Cell Proliferation
Clone Cells
DNA
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Genes, p53*
Heterografts
Humans
Mice
Mice, Nude
Neoplasm Metastasis
Parents
Phenotype*
Stomach Neoplasms*
Vascular Endothelial Growth Factor A
Adhesives
Agar
DNA
Vascular Endothelial Growth Factor A
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