Tuberc Respir Dis.  2006 Mar;60(3):290-296.

Analysis of DNA fingerprints of Mycobacterium Tuberculosis Isolates from Patients Registered at Health Center in Gyeonggi Province in 2004

Affiliations
  • 1Korean Institute of Tuberculosis, Seoul, Korea. gbai@hotmail.com
  • 2Hygiene Policy Department, Gyeonggi Provincial Government, Gyeonggi Province, Korea.
  • 3Ajou University School of Medicine, Gyeonggi Province, Korea.

Abstract

BACKGROUND: IS6110 DNA fingerprint is a very useful tool for investigating the transmission of tuberculosis. The aim of this study was to identify the epidemiological situations within a given area (one province).
METHODS
The 681 Mycbobacterium tuberculosis isolates from patients, who were registered at health centers in Gyeonggi Province from May to December in 2004, were subjected to IS6110 DNA fingerprinting. Patients belonging to clusters were interviewed by health-workers to determine their previous contacts or household TB history.
RESULTS
The number of IS6110 copies of the 681 isolates showed diverse fingerprint patterns from 0 to 21 of which the most prevalent copy number was 10 from 120 isolates (17.6%). Thirty-three isolates (4.8%) belonged to the K strain, and 128 isolates (18.8%) belonged to the K family. There were 180 (26.4%) isolates belonged belonging to fifty clusters, of which two clusters were within household transmission. Forty-three (23.9%) out of 180 patients resided in an area under the same health center control. The rate of clusters in those aged 60-70 was higher than in any other age group ( 95% CI of RR : 1.072 ~ 1.988).
CONCLUSION
This is the first report of an epidemiological survey based on a whole province using a DNA fingerprinting technique for M. tuberculosis. These results will be helpful in developing a program or policies to prevent the transmission of TB.

Keyword

Mycbobacterium tuberculosis; DNA fingerprinting; IS6110; Gyeonggi Province; Epidemiology; Cluster

MeSH Terms

Dermatoglyphics
DNA Fingerprinting*
DNA*
Epidemiology
Family Characteristics
Gyeonggi-do*
Humans
Mycobacterium tuberculosis*
Mycobacterium*
Tuberculosis
DNA

Figure

  • Figure 1 Number of M. tuberculosis isolates by IS6110 copies


Reference

1. World Health Organization. Global tuberculosis control surveillance, planning, financing: WHO report. 2005.
2. Korea Center for Disease Control and Prevention & Korean Institute of Tuberculosis. Annual report on the Notified Tuberculosis Patients in Korea. 2005.
3. van Soolingen D, Hermans PW, de Haas PE, Soll DR, van Embden JD. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991. 29:2578–2586.
4. van Soolingen D, Hermans PW, de Haas PE, van Embden JD. Insertion element IS1081-associated restriction fragment length polymorphisms in Mycobacterium tuberculosis complex species: a reliable tool for recognizing Mycobacterium bovis BCG. J Clin Microbiol. 1992. 30:1772–1777.
5. van Soolingen D, de Haas PE, Haagsma J, Eger T, Hermans PW, Ritacco V, et al. Use of various genetic markers in differentiation of Mycbacterium bovis strains from animals and humans and for studying epidemiology of bovine tuberculosis. J Clin Microbiol. 1994. 32:2425–2433.
6. Park YK, Bai GH, Kim SJ. Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from countries in the western pacific region. J Clin Microbiol. 2000. 38:191–197.
7. van Embden JD, Cave MD, Crawford JT, Dale JW, Eisenach KD, Gicquel B, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a stanard methodology. J Clim Microbiol. 1993. 31:406–409.
8. Herold CD, Fitzgerald RL, Herold DA. Current techniques in mycobacterial detection and speciation. Crit Rev Clin Lab Sci. 1996. 33:83–138.
9. van Soolingen D, Borgdorff MW, de Haas PE, Sebdk MM, Veen J, Dessens M, et al. Molecular epidemiology of tuberculosis in the Netherlands: a nationwide study from 1993 through 1997. J Infect Dis. 1999. 180:726–736.
10. Small PM, Hopewell PC, Singh SP, Paz A, Parsonnet J, Ruston DC, et al. The epidemiology of tuberculosis in San Francisco: a population-based study using conventional and molecular methods. N Engl J Med. 1994. 330:1703–1709.
11. Bifani PJ, Plikaytis BB, Kapur V, Stockbauer K, Pan X, Lutfey ML, et al. Origin and interstate spread of a New York City mutidrug-resistant Mycobacterium tuberculosis clone family. JAMA. 1996. 275:452–457.
12. Geng E, Kreiswirth B, Driver C, Li J, Burzynski J, DellaLatta P, et al. Changes in the transmission of tuberculosis in New York City from 1990 to 1999. N Engl J Med. 2002. 346:1453–1458.
13. Fujikane T, Fujiuchi S, Yamazaki Y, Matsumoto H, Takahashi M, Fujita Y, et al. Molecular epidemiology of tuberculosis in the north Hokkaido district of Japan. Int J Tuberc Lung Dis. 2004. 8:39–44.
14. Kim SJ, Bai GH, Lee H, Kim HJ, Lew WJ, Park YK, et al. Transmission of Mycobacterium tuberculosis among high school students in Korea. Int J Tuberc Lung Dis. 2001. 5:824–830.
Full Text Links
  • TRD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr