Nutr Res Pract.  2012 Oct;6(5):414-420.

Effect of coenzyme Q10 and Ardisia japonica Blume on plasma and liver lipids, platelet aggregation, and erythrocyte Na efflux channels in simvastatin-treated guinea pigs

• Kang MS
• Yang HM
• Kang JY
• Ryou SH
• Kang JS

Abstract

Forty guinea pigs were divided into four groups and fed 0.04% cholesterol based control diet, plus 0.05% simvastatin, and statin plus 0.1% CoQ10 or 10% Ardisia Japonica Blume (AJB) leave powder for 4 weeks. Plasma total cholesterol levels decreased significantly in all groups fed the statin-containing diet compared with that in guinea pigs fed the control diet (P < 0.01). Plasma and liver triglycerides decreased significantly in the statin plus CoQ10 group compared with those in the control (both P < 0.05). Maximum platelet aggregation was significantly higher in the statin plus CoQ10 group than that in the other groups (P < 0.05). Na-K ATPase activity increased in the statin group and decreased in the statin plus CoQ10 group (P < 0.01). Na-K co-transport and Na passive transport decreased significantly in the control group compared with those in the other groups (both P < 0.05). Intracellular Na was highest in the statin group and lowest in the statin plus CoQ10 group and was correlated with Na-K ATPase activity. Thiobarbituric acid reactive substance production in platelet-rich plasma and liver tended to decrease in the statin plus CoQ10 group compared with those in the other groups. Plasma glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase increased significantly in the statin group compared with those in the control (P < 0.05). These result suggest that antioxidant rich AJB did not have positive effects on cardiovascular disease parameters. The statin plus CoQ10 seemed to decrease cholesterol more efficiently than that of statin alone.

Keyword

Simvastatin; Ardisia japonica Blume; CoQ10; cholesterol; guinea pig

MeSH Terms

Adenosine Triphosphatases
Alanine Transaminase
Animals
Ardisia
Aspartate Aminotransferases
Blood Platelets
Cardiovascular Diseases
Cholesterol
Diet
Erythrocytes
Guinea
Guinea Pigs
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Liver
Plasma
Platelet Aggregation
Platelet-Rich Plasma
Simvastatin
Thiobarbiturates
Triglycerides
Ubiquinone
Adenosine Triphosphatases
Alanine Transaminase
Aspartate Aminotransferases
Cholesterol
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Simvastatin
Thiobarbiturates
Triglycerides
Ubiquinone

Figure

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  Fig. 1 Supplementation effects of simvastatin, coenzyme Q10 (CoQ10), and Ardisia japonica Blume (AJB) to cholesterol based diet on platelet aggregation and hematocrit. Values are mean ± SD of 10 guinea pigs.

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  Fig. 2 Supplementation effects of simvastatin, coenzyme Q10 (CoQ10), and Ardisia japonica Blume (AJB) to cholesterol based diet on erythrocyte sodium efflux channels. Values are mean ± SD of 10 guinea pigs.

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  Fig. 3 Supplementation effects of simvastatin, coenzyme Q10 (CoQ10), and Ardisia japonica Blume (AJB) to cholesterol based diet on platelet rich plasma (PRP) and liver thiobarbituric acid reactive substance (TBARS) production. Values are mean ± SD of 10 guinea pigs.

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  Fig. 4 Supplementation effects of simvastatin, coenzyme Q10 (CoQ10), and Ardisia japonica Blume (AJB) to cholesterol based diet on plasma glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) levels. Values are mean ± SD of 10 guinea pigs.

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