Mycobiology.
2014 Sep;42(3):256-261. 10.5941/MYCO.2014.42.3.256.
The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014
- Affiliations
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- 1Traditional and Biomedical Research Center, Daejeon University, Daejeon 300-716, Korea.
- 2Department of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Korea.
- 3Division of Life Sciences, College of Natural Science, Daejeon University, Daejeon 300-716, Korea. shinks@dju.kr
- KMID: 2312755
- DOI: http://doi.org/10.5941/MYCO.2014.42.3.256
Abstract
- A beta-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F2, and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside F2 and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract-->Rd-->F2-->compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively.
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