Mycobiology.  2010 Jun;38(2):128-132.

Fruit Body Formation on Silkworm by Cordyceps militaris

• Hong IP
• Kang PD
• Kim KY
• Nam SH
• Lee MY
• Choi YS
• Kim NS
• Kim HK
• Lee KG
• Humber RA

Abstract

Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a 100 microL injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than 2 x 105 colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.

Keyword

Bombyx mori; Cordyceps militaris; Fruit body; Hyphal bodies; Injection inoculation

MeSH Terms

Bombyx
Cordyceps
Fruit
Incidence
Light
Pupa
Stem Cells
Suspensions
Suspensions