Korean J Clin Pathol.  1999 Feb;19(1):114-117.

Evaluation of Genedia HBsAg Rapid and Genedia Anti-HBs Rapid for the Screening of HBsAg and Anti-HBs

• Oh J
• Kim TY
• Yoon HJ
• Min HS
• Lee HR
• Choi TY

Abstract

BACKGROUND
We evaluated a rapid screening kit for the detection of hepatitis surface antigen (HBsAg) and antibody (anti-HBs) using an immunochromatographic method.
MATERIALS AND METHODS
We selected 499 serum specimens for the evaluation. Each specimen was tested by enzyme immunoassay (EIA; Cobas Core, Roche, Switzerland), reverse passive hemagglutination (RPHA; Serodia HBs, Asan, Korea) for HBsAg, passive hemagglutination (PHA; Serodia Anti-HBs, Asan, Korea) for anti-HBs, and with the Genedia HBsAg and Anti-HBs Rapid (Green Cross Corp., Korea) kits. Results of each assay were compared with those of the EIA.
RESULTS
The sensitivities and specificities of the Genedia HBsAg Rapid kit were 98.0% and 100%, and those for the Genedia Anti-HBs Rapid kit were 95.3% and 98.0%, respectively. These were higher than those for RPHA (96.0% and 100%), and PHA (83.2% and 96.0%). Concordance rates between EIA and Genedia HBsAg Rapid, Genedia Anti-HBs Rapid, RPHA, and PHA were 98.8%, 96.4%, 97.6%, and 88.4%, respectively. Extending the incubation time from the recommended 30 minutes to 2 hours increased the sensitivities of the Genedia kits.
CONCLUSION
The Genedia HBsAg and Anti-HBs Rapid kits are simple, sensitive, and inexpensive assays suitable for screening or use in emergency situations.

Keyword

HBsAg; Anti-HBs; Screening kit; Genedia HBsAg Rapid; Genedia Anti-HBs Rapid

MeSH Terms

Antigens, Surface
Chungcheongnam-do
Emergencies
Hemagglutination
Hepatitis
Hepatitis B Surface Antigens*
Immunoenzyme Techniques
Mass Screening*
Antigens, Surface
Hepatitis B Surface Antigens