Korean J Blood Transfus.  1997 Dec;8(2):97-101.

Fetal D positive blood detection by PCR in D negative preganant

• Lim CS
• Lee KN
• Park YK

Abstract

BACKGROUND
Presence of fetal D positive RBCs in maternal peripheral blood of D negative pregnants induce Rh alloimmunization. Early detection of fetal D positive RBCs would eliminate the risk to an D positive fetus in pregnancy. We demonstrate and evaluate relibility of a sensitive polymerase chain reaction(PCR)-based assay for the RHD fetus gene detection from maternal peripheral blood samples.
METHODS
The amplification of RHD gene and RHCcEe gene site were done in peripheral blood sample(n=17) of nine D negative pregnants by polymerase chain reaction and evaluated the sensitivity of PCR genotyping in serially diluted D positive sample. If RHD amplicated band(189 bp) were found with RHCcEe band(136 bp) in peripheral blood of D negative pregnants, the results were interpreted as positive for fetomaternal hemorrhage detection.
RESULTS
Gestational age distribution of samples were from 7 to 39 weeks include postpartum one day. The RHD typing by PCR showed good sensitivity to detect up to 0.05% fetomaternal hemorrhage. Two cases were positive by PCR among 17 samples and their gestaional age was 22 and 27 weeks.
CONCLUSIONS
RHD PCR showed good sensitivity for fetomaternal hemorrhage detection and prophylaxis of Rho(D) alloimmunization should be carefully done by this results because fetomaternal hemorrhage were detected in early gestational age by PCR.

Keyword

Rho(D); Polymerase Chain Reaction; Fetomaternal hemorrhage

MeSH Terms

Female
Fetomaternal Transfusion
Fetus
Gestational Age
Polymerase Chain Reaction*
Postpartum Period
Pregnancy