Korean J Blood Transfus.  2006 Jun;17(1):77-81.

Two Cases of Anti-M of Donated Bloods Confirmed at pH 6.5

• Chang SL
• Kim JP
• Choi BC
• Chung HR
• Oh DJ

Abstract

Anti-M is detected at room temperature and is often found in the sera of people who have never been exposed to human red cells. In a few cases, anti-M can be detected at 37 degrees C or at the antiglobulin phase, and these antibodies can cause hemolytic diseases in newborn or hemolytic transfusion reactions. Some examples of anti-M demonstrate stronger agglutination at low pH (pH 6.5), and when they react with the red blood cells of the MM type (dosage effect). An unexpected antibody test was carried out for the routine screening of donated blood and two cases that reacted to all panel cells at 5 degrees C were found, which indicated anti-M. We repeated the unexpected antibody identification test at pH 6.5 and confirmed the presence of anti-M. The reduction of the test system pH is a useful and simple method for detecting some cases of anti-M.

Keyword

Anti-M antibody; pH 6.5

MeSH Terms

Agglutination
Antibodies
Blood Group Incompatibility
Erythrocytes
Humans
Hydrogen-Ion Concentration*
Infant, Newborn
Mass Screening
Antibodies