Korean J Urol.
1998 Sep;39(9):827-831.
Effect of Oxalate on the Growth of Distal Renal Tubular Epithelial Cells
- Affiliations
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- 1Department of Urology, College of Medicine, Seoul National University, Seoul, Korea.
Abstract
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PURPOSE: We tried to evaluate the effect of oxalate on the growth of distal renal tubular epithelial cells
MATERIALS AND METHODS
Madin-Darby canine kidney(MDCK) cell line, derived from canine distal renal tubular epithelial cell, supplied from American Type Culture Collection was used in this study. RPMI 1640 containing 10mM HEPES-buffer, 100IU/ microliterpenicillin, 100 microgram/ microliterstreptomycin, 300 microgram/ microliterglutamine, and with or without 10% fetal bovine serum(FBS) was used as the culture media. And the cell survivals under various concentrations of ammonium oxalate were assessed by MTT assay.
RESULTS
Exposure of MDCK cells to oxalate resulted in a concentration dependent suppression of the cell growth in both 10% FBS supplied media and FBS deprived media.
CONCLUSIONS
From these observations we could conclude that oxalate is not a mere constituent of calcium oxalate stone, but oxalate might offer a good environment for calcium oxalate stone formation in the urinary tract via MDCK cellular damage.
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