Korean J Physiol Pharmacol.  2006 Jun;10(3):123-129.

Estrogen Pretreatment of Organotypic Hippocampal Slices Protects Neurons against Oxygen-Glucose Deprivation with Akt Activation

Affiliations
  • 1Department of Pharmacology, College of Medicine, Ewha Womans University, Seoul, Korea. empark@ewha.ac.kr

Abstract

In several experimental models, estrogens protect neurons against ischemic insults. However, the recent clinical studies of hormone replacement showed negative results to prevent stroke. Therefore, optimal models to study estrogen replacement for neuroprotection are needed before its clinical application. Organotypic hippocampal slice under oxygen-glucose deprivation (OGD) has been established as a model of cerebral ischemia and has advantages to study drug effects. We investigated whether estrogen protected CA1 neurons and affected activation of Akt (pAkt) in CA1 region under OGD. Thus, rat hippocampal slices on day 7 of culture were treated with 17-beta estradiol (E, 1 nM) for 7 days before 30 min OGD, and cell death of CA1 neurons was quantified by propidium iodide (PI) staining and expression of pAkt was studied by Western blot and immunofluorescence. PI intensity in slices treated with E was significantly reduced 72 hour after OGD compared to that of non-treated slices (p<0.05). E pretreatment also increased the expression of pAkt 72 hour after OGD compared to that of no treatment (p<0.01). These data suggest that estrogen pretreatment may rescue neurons from ischemic insults through the activation of Akt and also indicate that our model would be a useful alternative method to study the mechanisms and effects of estrogen replacement treatment for neuroprotection.

Keyword

Estrogen; Pretreatment; Akt activation; OGD; Organotypic hippocampal slice; Neuroprotection

MeSH Terms

Animals
Blotting, Western
Brain Ischemia
Cell Death
Estradiol
Estrogen Replacement Therapy
Estrogens*
Female
Fluorescent Antibody Technique
Models, Theoretical
Neurons*
Propidium
Rats
Stroke
Estradiol
Estrogens
Propidium
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