Korean J Otolaryngol-Head Neck Surg.  2001 Aug;44(8):853-858.

An Immunohistochemical Tracing on the Central Neural Pathways of the Spinal Accessory Nerve using Pseudorabies Virus

• Park JJ
• Hwang EG
• Jung TG
• Kim JP
• Jeon SY

Abstract

BACKGROUND AND OBJECTIVE: The transsynaptic transfer of neurotropic viruses is an effective tool for tracing chains of connected neurons because the replication of virus in the recipient neurons after transfer amplifies the "tracer signal". The purpose of study was to identify the location of spinal nucleus of the accessory nerve and the central neural pathways using Bartha strain of Pseudorabies virus (PRV-Ba), as a transsynaptic tracer.
MATERIALS AND METHODS
PRV-Ba was injected into the sternocleidomastoid muscle of a rat, and the localization of PRV-Ba in the rat spinal cord and CNS was identified with light microscopic immunohistochemistry using primary antibodies against the PRV-Ba.
RESULTS
Sequential tracing of retrogradely labeled cells was done. The shapes of positive immunoreactive cells were mostly ovoid or polygonal, and were shown in the spinal nucleus of the accessory nerve, nucleus ambiguus, paraventricular nucleus, and the primary motor area of cerebral cortex.
CONCLUSIONS
These results showed the location of spinal accessory nucleus and the central neural pathways of spinal accessory nerve using PRV-Ba.

Keyword

Pseudorabies virus; Spinal accessory nerve; Immunohistochemistry

MeSH Terms

Accessory Nerve*
Animals
Antibodies
Cerebral Cortex
Herpesvirus 1, Suid*
Immunohistochemistry
Neural Pathways*
Neurons
Paraventricular Hypothalamic Nucleus
Pseudorabies*
Rats
Spinal Cord
Antibodies