Korean J Obstet Gynecol.  2009 Dec;52(12):1265-1272.

The relationship between serum human papillomavirus 16 DNA and clinicopathological prognostic factors in cervical cancer patients

• Lee ES
• Jeong DH
• Kim JR
• Lee DS
• Kim MS
• Cho YK
• Byun JM
• Park YM
• Kim YN
• Jeong CH
• Lee KB
• Sung MS
• Kim KT

Abstract

OBJECTIVE
To study the relationship between serum human papillomavirus (HPV) deoxyribonucleic acid (DNA) and clinicopathologic prognostic factors and the clinical usefulness of serum HPV 16 DNA in cervical cancer patients.
METHODS
All the patients were treated at our institution, from January, 2002 to February, 2007. DNA extracted from serum of 17 patients with HPV 16 infected carcinoma in situ and 65 patients with HPV 16 infected squamous cell carcinoma of the uterine cervix (stage IA-IIIB) were examined for HPV 16 DNA using polymerase chain reaction with types 16 specific E6 primer. Clinicopathological parameters were obtained from medical records, and the relationship between the discrete variables and serum HPV 16 DNA status were evaluated.
RESULTS
HPV 16 DNA was not detected in serum from all patients with carcinoma in situ. However, among the 65 patients with HPV 16 infected squamous cell carcinoma of the uterine cervix, we detected 17 HPV 16 DNA positive samples (26.2%) in serum. Positive HPV 16 DNA in serum was correlated with age (P=0.0071), serum squamous cell carcinoma (SCC) antigen (P=0.0034), tumor size (P=0.0029), clinical stage (P<0.0001), deep stromal invasion (P=0.0048), resection margin positivity (P=0.0008), and pelvic lymph nodal metastasis (P=0.0040).
CONCLUSION
The serum HPV 16 DNA in patients with cervical cancer was correlated with poor prognostic factors that need adjuvant treatment.

Keyword

Cervical cancer; Human papillomavirus; Serum

MeSH Terms

Carcinoma in Situ
Carcinoma, Squamous Cell
Cervix Uteri
DNA
Female
Human papillomavirus 16
Humans
Medical Records
Neoplasm Metastasis
Polymerase Chain Reaction
Uterine Cervical Neoplasms
DNA