Korean J Obstet Gynecol.
2005 May;48(5):1265-1270.
Effect of exogenous gonadotropin on HB-EGF expression in endometrium
- Affiliations
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- 1Department of Obstetrics and Gynecology, College of Medicine, Medical Research Center, Seoul National University, Korea. ymchoi@snu.ac.kr
- 2Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Korea.
- 3Department of Obstetrics and Gynecology, Eulji University School of Medicine, Korea.
Abstract
OBJECTIVE
Gonadotropin hyperstimulation for in vitro fertilization and embryo transfer (IVF-ET) has been considered to be harmful to the uterine receptivity. The role of HB-EGF has been implicated in the mouse embryonic implantation. In the present study, we assessed the effect of gonadotropin hyperstimulation on the expression of HB-EGF mRNA in murine endometrium.
METHODS
Competitive, reverse transcription-PCR was performed to evaluate the expression of HB-EGF mRNA in the hyperstimulated murine endometrum from pregnant uteri of gestation day (g.d.) 5.5 after the injection of saline (placebo), pregnant mare serum gonadotropin (PMSG) 5 IU and 10 IU.
RESULTS
HB-EGF expression was observed at 10-4-10-6 dilution concentrations of internal standard, and dual bands (423/360 bp) were observed at 10-5 dilution concentration. The expression of HB-EGF mRNA showed no significant change in the endometrial expression in PMSG groups, compared to the control group, on g.d. 5.5. There was no dose-response relationship between the two PMSG groups.
CONCLUSION
Our data reveals that ovarian hyperstimulation using gonadotropin does not influence the expression of HB-EGF in the murine endometrium. Further studies are necessary to elucidate which stage of embryonic implantation and the expression of other substance is affected by the ovarian hyperstimulation.
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