Blood Res.  2013 Mar;48(1):51-54. 10.5045/br.2013.48.1.51.

Blood group phenotype frequencies in blood donors from a tertiary care hospital in north India

• Agarwal N
• Thapliyal
• Chatterjee K

• KMID: 2270727
• DOI: http://doi.org/10.5045/br.2013.48.1.51

Abstract

BACKGROUND
Knowledge about the frequency of red blood cell-antigen phenotypes in a population can be helpful in the creation of a donor data bank for the preparation of indigenous cell panels and for providing antigen-negative compatible blood to patients with multiple alloantibodies.
METHODS
ABO and RhD blood grouping was performed on 9,280 continuous voluntary and replacement donors. For other rare blood groups, 508 ACD blood samples were obtained from the donors at the Blood Bank of the Department of Transfusion Medicine, All India Institute of Medical Sciences (AIIMS), New Delhi, India. Blood group antigens were determined by tube method using anti-sera (Bio-Rad, USA), and the phenotype frequencies were expressed as percentages.
RESULTS
Group B (37.39%) was the most common, followed by group O (31.85%). R1R1 and rr were the most common phenotypes amongst Rh positive and Rh negative groups, respectively. A rare phenotype R2Rz was found in one donor. For Kidd and Duffy blood group systems, Jk (a+b+) and Fy (a+b+) were the most common phenotypes (46.06% and 48.03%, respectively). The most common phenotypes for MNSs, Lu, and Kell blood groups were M+N+, S-s+, Lu (a-b+), and K-k+, respectively. A very rare case of Fy (a-b-) and Jk (a-b-) was found in a single donor.
CONCLUSION
This study is the first small step to create a rare donor data bank and to prepare indigenous cell panels to provide compatible blood to all multi-transfused alloimmunized patients.

Keyword

Blood group; MNS; ABO; Rh; Kell; Kidd; Duffy

MeSH Terms

Blood Banks
Blood Donors
Blood Group Antigens
Blood Grouping and Crossmatching
Duffy Blood-Group System
Humans
India
Phenotype
Tertiary Healthcare
Tissue Donors
Blood Group Antigens
Duffy Blood-Group System

Reference

1. Cartron JP, Colin Y. Structural and functional diversity of blood group antigens. Transfus Clin Biol. 2001; 8:163–199. PMID: 11499957.

2. Reid ME. ISBT science series - ISBT scientific series - State of the art invited speaker. Protein blood group systems: Berlin and beyond. 2012; Vol. 7:41–43.

3. Sadeghian MH, Keramati MR, Badiei Z, et al. Alloimmunization among transfusion-dependent thalassemia patients. Asian J Transfus Sci. 2009; 3:95–98. PMID: 20808654.

4. Nanu A, Thapliyal RM. Blood group gene frequency in a selected north Indian population. Indian J Med Res. 1997; 106:242–246. PMID: 9378531.

5. Beadling WV, Cooling L. McPherson RA, Pincus MR, editors. Immunohematology. Henry's clinical diagnosis and management by laboratory methods. 2007. 21st ed. Philadelphia, PA: Saunders Elsevier;p. 617–664.

6. Thakral B, Saluja K, Sharma RR, Marwaha N. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in north Indian blood donors. Transfus Apher Sci. 2010; 43:17–22. PMID: 20558108.

7. Nathalang O, Kuvanont S, Punyaprasiddhi P, Tasaniyanonda C, Sriphaisal T. A preliminary study of the distribution of blood group systems in Thai blood donors determined by the gel test. Southeast Asian J Trop Med Public Health. 2001; 32:204–207. PMID: 11485086.

8. Cleghorn TE. MNSs gene frequencies in English blood donors. Nature. 1960; 187:701. PMID: 13810614.

9. Chandra T, Gupta A. Frequency of ABO and rhesus blood groups in blood donors. Asian J Transfus Sci. 2012; 6:52–53. PMID: 22623849.

10. Fischbach F. Nurses' quick reference to common laboratory and diagnostic tests. 2002. 3rd ed. Philadelphia, PA: Lippincott;p. 19–25.

11. Avent ND, Reid ME. The Rh blood group system: a review. Blood. 2000; 95:375–387. PMID: 10627438.

12. Daniels G. Human blood groups. 2002. 2nd ed. Hoboken, NJ: John Wiley & Sons, Inc..

13. Hillyer CD, Shaz BH, Zimring JC, Abshire TC, editors. Transfusion medicine and hemostasis. 2009. Philadelphia, PA: Elsevier;p. 139–144.

14. Reid ME, Lomas-Francis C. The blood group antigen facts book. 2004. 2nd ed. San Diego, CA: Academic Press;p. 265–277.