Korean J Obstet Gynecol.  2001 Aug;44(8):1514-1521.

Inhibitory effect of Nitric oxide on the Proliferation of Endometrial cells

Affiliations
  • 1Department of Obstetrics & Gynecology, College of Medicine, Pusan National University, Pusan, Korea..

Abstract


OBJECTIVE
Development of endometriosis is associated with endometrial stromal cell proliferation. The effects of nitric oxide (NO) on the proliferation of endometrial cells and the effects of peritoneal fluid from women with and without endometriosis on the production of NO and NO-induced changes were investigated.
METHODS
Endometrial stromal cells were prepared from women with endometriosis (n=10) and healthy control (n=10). Peritoneal fluid from 10 patients were collected at the beginning of laparoscopic procedures. The proliferation of cells was assessed by [3H]thymidine. Nitrite, a stable NO product, was measured with Griess reagents. Expression level of endothelial nitric oxide synthase (eNOS) was analyzed by quantitative RT-PCR.
RESULTS
Sodium nitroprusside, an NO donor, reduced proliferation of the endometrial and endometriosis cells from primary culture in a dose-dependent manner. The increased production of NO and inhibitory effect of NO on the endometrial stromal cell proliferation were reduced by peritoneal fluid from patients with endometriosis but not by interleukin-8, 17-beta estradiol or TGF-beta1. Transcripts of the endothelial NOS (eNOS) gene were detected in unstimulated endometrial cells and the expression patterns were not changed by exposure of cells to SNP or peritoneal fluid for 24 hours.
CONCLUSION
It is postulated that these findings show NO inhibits proliferation of endometrial cells and the inhibitory effect of NO on endometrial cells was abrogated by certain factor (s) of peritoneal fluid from patients with endometriosis. In conclusion, NO appears to be involved in the pathogenesis of endometriosis.

Keyword

Endometrial cell; Nitric oxide; Peritoneal fluid

MeSH Terms

Ascitic Fluid
Endometriosis
Estradiol
Female
Humans
Indicators and Reagents
Interleukin-8
Nitric Oxide Synthase Type III
Nitric Oxide*
Nitroprusside
Stromal Cells
Tissue Donors
Transforming Growth Factor beta1
Estradiol
Indicators and Reagents
Interleukin-8
Nitric Oxide
Nitric Oxide Synthase Type III
Nitroprusside
Transforming Growth Factor beta1
Full Text Links
  • KJOG
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr