Korean J Hepatobiliary Pancreat Surg.  2004 Sep;8(3):172-179.

Cyclooxygenase-2 Expression in Bile Duct Cancer

Affiliations
  • 1Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. sunkim@plaza.snu.ac.kr
  • 2Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
  • 3Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
  • 4Department of Surgery, Cheju National University College of Medicine, Jeju, Korea.

Abstract

PURPOSE
Epidemiological studies have shown that regular ingestion of a nonsteroidal anti-inflammatory drug reduces the risk of fatality from colon cancer. Cyclooxygenase-2 (COX-2) has been found to be overexpressed in various types of tumor, including colorectal cancer. It has been suggested that the COX-2 enzyme may play an important role in carcinogenesis and tumor progression. The aims of this study were to find the relationship between COX-2 expression and bile duct carcinogenesis and its clinical significance in bile duct cancer. METHODS: The COX-2 expression was determined using three methods, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining. mRNA and proteins were extracted from the tissue specimens of 22 bile duct cancer patients. RESULTS: RT-PCR and western blotting found COX-2 expressions in all the cancers and their paired noncancerous tissues. Immunohistochemistry revealed the highest levels of COX-2 expressed in bile duct carcinoma cells, mainly in the cytoplasm, and a weak reactivity of the COX-2 protein was observed in noncancerous bile duct epithelial cells. CONCLUSION: Based on this study, it is postulated that the carcinogenesis mechanism of COX-2 expression may be initiated from a noncancerous condition, such as chronic inflammation, and may advance to bile duct cancer progression.

Keyword

Bile Duct Neoplasms; Cyclooxygenase; Immunohistochemistry; Western Blotting

MeSH Terms

Bile Duct Neoplasms*
Bile Ducts*
Bile*
Blotting, Western
Carcinogenesis
Colonic Neoplasms
Colorectal Neoplasms
Cyclooxygenase 2*
Cytoplasm
Eating
Epithelial Cells
Humans
Immunohistochemistry
Inflammation
Polymerase Chain Reaction
Prostaglandin-Endoperoxide Synthases
Reverse Transcription
RNA, Messenger
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
RNA, Messenger
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