J Korean Ophthalmol Soc.  2013 Jan;54(1):149-154. 10.3341/jkos.2013.54.1.149.

Inflammatory Cytokines in the Vitreous of Rabbits after Photocoagulation Using Pattern Scanning and Conventional Laser

• Choi YR
• Byon IS
• Lee SU
• Shin MK
• Lee JE
• Oum BS

• KMID: 2216464
• DOI: http://doi.org/10.3341/jkos.2013.54.1.149

Abstract

PURPOSE
To compare concentration of cytokines in the vitreous of rabbit eyes after photocoagulation using a short-pulse pattern scanning laser (PASCAL) or a conventional laser.
METHODS
Laser photocoagulation was performed using PASCAL (duration 0.02 seconds) in the right eyes and a conventional laser (duration 0.1 seconds) in the left eyes of 13 pigmented rabbits. To obtain ophthalmoscopically similar mild burns, power was adjusted during the photocoagulation. The rabbits were sacrificed at 1, 3 or 7 days after photocoagulation to investigate histological changes. Levels of interleukins (IL)-1beta, IL-6, IL-8 and tumor necrosis factor (TNF)-alpha in the vitreous humors of ten rabbits were measured using enzyme-linked immunosorbent assay before treatment and at 1, 3 and 7 days after photocoagulation.
RESULTS
Histological changes were comparable between the two groups. IL-1beta and TNF-alpha were not detectable. IL-6 did not change significantly. IL-8 increased at day 3 and day 7, but no significant difference was observed between the two groups (p>0.05).
CONCLUSIONS
When ophthalmoscopically similar mild burn intensity was achieved, conventional and PASCAL treatments of rabbit eyes caused no difference in intravitreal concentration of inflammatory cytokines.

Keyword

Cytokine; Interleukin; Patterned scanning laser; Photocoagulation

MeSH Terms

Burns
Cytokines*
Enzyme-Linked Immunosorbent Assay
Interleukin-6
Interleukin-8
Interleukins
Light Coagulation*
Rabbits*
Tumor Necrosis Factor-alpha
Vitreous Body
Cytokines
Interleukin-6
Interleukin-8
Interleukins
Tumor Necrosis Factor-alpha

Figure

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  Figure 1. Ophthalmoscopic findings after photocoagulation using pattern scanning laser (A: day 0; C: day 1; E: day 3; G: day 7) and conventional laser (B: day 0; D: day 1; F: day 3; H: day 7).

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  Figure 2. Microscopic findings after photocoagulation using pattern scanning laser (A: day 1; C: day 3; E: day 7) and conventional laser (B: day 1; D: day 3; F: day 7).

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  Figure 3. Intravitreal concentration of interleukin-6 (IL-6) after photocoagulation using pattern scanning laser and conventional laser. Each symbol represents the lowest, the average and the highest concentration.

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  Figure 4. Intravitreal concentration of interleukin-8 (IL-8) after photocoagulation using pattern scanning laser and conventional laser (* p<0.05, compared to baseline). Each symbol represents the lowest, the average and the highest concentration.

Reference

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