J Korean Pediatr Soc.  2000 Feb;43(2):179-187.

Construction and Application of Painting probe for Chromosome band 5q14-q15, 6q23, 16p11.2 and 22q12

• Park SY
• Park SY
• Kil JI
• Chun YH
• Park SH

Abstract

PURPOSE: Chromosome microdissection has become a very powerful approach to generate chromosome band-specific library and painting probes for physical mapping or cytogenetic analysis. We have constructed here band-specific painting probes for human chromosomes by microdissection and polymerase chain reaction(PCR).
METHODS
We pretreated the microdissected fragments with Topoisomerase I(Topo I) which catalyzes the relaxation of supercoiled DNA, and performed two initial rounds of DNA synthesis with T7 DNA polymerase followed by conventional PCR to enable the reliable preparation of fluorescent in situ hybridization(FISH) probe.
RESULTS
It was possible to construct region-specific painting probes for 5q14-q15, 6q23, 16p11.2 and 22q12. The painting probes were successful in determining the corresponding chromosome bands in SNU-484 cell line, IMR-32 cell line.
CONCLUSION
These painting probes can be used to detect the related marker chromosomes in congenital malformations and other solid tumors, and the band specific probe pools may be used effectively to analyse the genes.

Keyword

Chromosome microdissection; PCR; Band-specific painting probe; FISH

MeSH Terms

Cell Line
Chromosomes, Human
Cytogenetic Analysis
DNA
DNA, Superhelical
Humans
Microdissection
Paint*
Paintings*
Polymerase Chain Reaction
Relaxation
DNA
DNA, Superhelical