J Lab Med Qual Assur.  2010 Dec;32(2):255-262.

Arg72Pro Polymorphism and Exon 7 Codon 249 Mutation of Plasma DNA p53 Gene in Early Hepatocellular Carcinoma Patients with Hepatitis B Virus Infection in a Korean population

• Park Y
• Lee JH
• Lee EY
• Kim HS

Abstract

BACKGROUND
This study was performed to investigate on the genotypic frequencies of p53 Arg72Pro polymorphism and the prevalence of p53 codon 249 mutation in hepatocellular carcinoma patients.
METHODS
Plasma DNAs were extracted from the samples of 44 early HCC cases, 24 chronic B-viral hepatitis patients and 27 healthy individuals. Serum levels of AFP, PIVKA-II, and HBV DNA-positive rates among the study groups were also compared. PCR-based restriction fragment length polymorphism method was used to determine p53 Arg72Pro genotype and to detect codon 249 mutation.
RESULTS
Serum AFP and PIVKA-II level, Edmondson grade, tumor size and frequency of HBV DNA-positivity among HCC group according to Arg72Pro genotypes showed no statistically significant difference. The frequencies of Arg72Pro genotypes (Arg/Arg, Arg/Pro, Pro/Pro) were respectively as follows: 34.1%, 47.7%, 18.2% in HCC group; 29.2%, 54.2%, 16.7% in hepatitis group; 29.6%, 55.6%, 14.8% in control group. Pro homozygote genotype had a higher risk for developing HCC by adjusted OR (1.529, 95% CI 0.325-7.193), but not statistically significant (P=0.591). No codon 249 mutation was found among 44 HCC cases.
CONCLUSIONS
Pro homozygote was around 16% in all study groups, and did not statistically increase risks to developing HCC. We suggest that Arg72Pro polymorphism of p53 gene is not a significant risk factor in early hepatocarcinogenesis.

Keyword

Hepatocellular carcinoma; p53; Hepatitis B virus; Polymorphism

MeSH Terms

Biomarkers
Carcinoma, Hepatocellular
Codon
DNA
Exons
Genes, p53
Genotype
Hepatitis
Hepatitis B
Hepatitis B virus
Homozygote
Humans
Plasma
Polymorphism, Restriction Fragment Length
Prevalence
Protein Precursors
Prothrombin
Risk Factors
Codon
DNA
Protein Precursors
Prothrombin