J Korean Neurosurg Soc.
1998 Jul;27(7):872-882.
Patterns of Invasion in Rat Gliosarcoma
- Affiliations
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- 1Department of Neurosurgery, College of Medicine, Korea University, Seoul, Korea.
Abstract
- The aim of this study was to investigate the patterns of invasion into the surrounding normal brain tissue in malignant gliomas. The C9L gliosarcoma cell line was used to produce a tumor in the concentration of 2X10 6 cells/microL. The experimental animals, male Sprague-Dawley rats, were anesthetized and secured in a stereotactic frame for left parietal craniectomy and tumor cell injection, and sacrificed in 10 to 14 days. The authors evaluated the patterns of tumor growth such as mass size, invasion site, formation of secondary structures, type of necrosis, and evolvement of blood vessels using routine H-E stain, Miller's elastic stain and immunohistochemical stain for vascular endothelial growth factor(VEGF). The tumor was produced in 8 of 16 cases, which were mostly consisted of malignant spindle cells. Regarding the tumor growth patterns, infiltrative type was observed more frequently than expansion type. Leptomeningeal involvement was observed in 6 cases which showed infiltrative growth pattern, and ventricular dissemination was noted in 3 cases and choroid plexus involvement was found in 1 case. Secondary tumor structures such as perivascular tumor cell aggregates and separate tumor micronodules were observed in 4 cases and 6 cases, respectively. Three types of necrosis were developed in the center of tumor, such as geographic (2 cases), hemorrhagic(2 cases) and ischemic necrosis(1 case). Based on the histologic findings in this experiment, it is presumed that the leptomeningeal dissemination and formation of secondary structures around the primary tumor site might play a significant role in the distant metastasis and recurrence.
