J Korean Acad Conserv Dent.  2002 Jan;27(1):34-43. 10.5395/JKACD.2002.27.1.034.

Microleakage and marginal hybrid layer of dentin adhesives

• Cho YG
• Kim YK
• Ahn JM

• KMID: 2175546
• DOI: http://doi.org/10.5395/JKACD.2002.27.1.034

Abstract

The purpose of this study was to compare microleakage and marginal hybrid layer in class V restorations using two one-bottle adhesives and one self-etching adhesive. Class V cavity preparations with occlusal margins in enamel and gingival margins in dentin were prepared on buccal and lingual surfaces of 30 extracted human molar teeth. Prepared teeth were randomly divided into three treatment groups (n=30) and restored with three adhesives and composites: Single Bond/Filtek Z-250 (Group 1), Prime&BondNT/Esthet.X (Group 2), UniFil Bond/UniFil F (Group 3). For microleakage, samples were stored in room temperature water for 24 hours, thermocycled, stained with 2% methylene blue dye, sectioned into halves, scored and analysed using Mann-whitney test and Wilcoxon signed rank sum test. For marginal hybrid layer, samples were sectioned into halves, treated with 10% phosphoric acid for 5 seconds, stored in 5% NaOCL solution for 24 hours, dried and gold coated. Occlusal and gingival margins of each sample were inspected under SEM. The results of this study were as follows; 1. Microleakage at the occlusal margins was not evident in group 1 and group 2, but it showed in group 3 (p<0.05). 2. Microleakage in group 1 and group 3 was significantly lower than in group 2 at gingival margins (p<0.05). 3. Microleakage at gingival margins was greater than at occlusal margins in group 1 and group 2, but microleakage at occlusal margins was greater than at gingival margins in group 3 (p<0.05). 4. In group 1 and group 2, no gaps at occlusal margins showed. But gaps showed in group 3. Occlusal margins were free from a hybrid layer in all groups. 5. The thickness of the marginal hybrid layers was 2.5~5 microm thick in group 5 microm thick in group 2 and 1.5 microm thick in group 3. 6. There was no corelation between microleakage and thickness of marginal hybrid layer. In coclusion, the effect of dentin adhesives on microleakge in class V composite restorations was excellent when one-bottle adhesives were applied on enamel margin, and it was good when a self-etching adhesive was applied on dentinal margin. There was no corelation between microleakage and thickness of marginal hybrid layer.

MeSH Terms

Adhesives
Chimera
Dental Enamel
Dentin
Humans
Methylene Blue
Molar
Phosphoric Acids
Tooth
Water
Adhesives
Methylene Blue
Phosphoric Acids
Water

Figure

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  Fig. 1. Microleakage in group 1 (Single Bond/Filtek Z-250) Microleakage did not showed at occlusal margin(Do). Microleakage of degree 1(D1) was showed at gingival margin (× 15).

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  Fig. 2. Microleakage in group 2 (Prime&Bond NT/Esthet∙X) Microleakage did not showed at occlusal margin(Do). Microleakage of degree 3(D3) showed at gingival margin (× 15).

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  Fig. 3. Microleakage in group 3 (UniFil Bond/UniFil F) Microleakage of degree 2(D2) showed at occlusal margin. Microleakage of degree 1(D1) showed at gingival margin (× 15).

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  Fig. 4. SEM of occlusal margin in group 1 (× 500) Close adaptation between interface of resin(R) and enamel(E) was evident and no gaps showed at occlusal margins. Hybrid layer was free.

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  Fig. 5. SEM of occlusal margin in group 2 (× 500) Close adaptation between interface of resin(R) and enamel(E) was evident and no gaps showed at occlusal margins. Hybrid layer was free.

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  Fig. 6. SEM of occlusal margin in group 3 (× 500) Tiny gaps(g) between interface of resin(R) and enamel(E) showed. Hybrid layer was free.

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  Fig. 7. SEM of gingival margin in group 1 (× 500) Thickness of the marginal hybrid layers(h) was 2.5∼5μm and tiny gaps(g) between interface of resin(R) and detin(D) showed.

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  Fig. 8. SEM of gingival margin in group 2 (× 500) Thickness of the marginal hybrid layers(h) was 5μm and wide gaps(g) between interface of resin(R) and dentin(D) showed.

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  Fig. 9. SEM of gingival margin in group 3 (× 500) Thickness of the marginal hybrid layers(h) was 1.5μm and tiny gaps(g) between interface of resin(R) and dentin(D) showed.

Reference

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