J Korean Cancer Assoc.
2000 Jun;32(3):563-570.
Sequential Changes of Proliferative Fraction of Enzyme Altered
Fdegrees Ci in Experimental Rat Hepatdegrees Carcinogenesis
- Affiliations
-
- 1Department of Pathology, Seoul National University College of Medicine.
- 2Department of Pathology, Ulsan University College of Medicine.
- 3Seoul National University Hospitial, Clinical Research Institute, Seoul, Korea.
Abstract
-
PURPOSE: The proliferative activity of cells in enzyme altered fdegrees Ci of the rat hepatoma model
was measured by double immunohistdegrees Chemical staining methods using anti-bromodeoxyuridine
(BrdU) and anti-glutathione S transferase of placental form (GST-P). The aim of this study was
to compare the cell proliferative activity in GST-P positive altered fdegrees Ci and in negative fdegrees Ci.
MATERIALS AND METHODS
Eight-week-old male Sprague-Dawley rats were administered by 200
mg/kg diethylnitrosamine (DEN) intraperitoneally, and followed by 0.02% acetylaminofluorene
(AAF)-containing diet for 4 weeks. One week after administration of AAF diet, two-thirds hepa
tectomy was performed. Control animals were treated as same except for the omission of AAF
in the diet. The rats were sacrified 0, 1, 3, 5, 7, 14 and 21 days after partial hepatectomy. The
slices of liver were fixed in acetone, dehydrated in benzene and stained by peroxidase-anti
peroxidase method against GST-P and by avidine-biotin peroxidase complex method against BrdU.
RESULTS
The area of the GST-P positive fdegrees Ci was increased during the experimental period. In
the experimental group, the S-phase fraction in the fdegrees Ci remained high during the first week and
was decreased thereafter. However, the GST-P negative area maintained a low S-phase cell frac
tion throughout the experimental period.
CONCLUSION
These results suggest that hepatic cells in the enzyme altered fdegrees Ci may escape a
suppressor effect of AAF in contrast to the normal cells in which their growth are inhibited by
AAF.