J Breast Cancer.  2007 Mar;10(1):29-35. 10.4048/jbc.2007.10.1.29.

The Multidrug Resistance-related Protein and P-glycoprotein Expressions, and the Washout Rates of 99mTc-MIBI in Infiltrating Ductal Carcinoma of Breast, Correlation with the Response After Neoadjuvant Chemotherapy

• Kwak HS
• Bae YT
• Han KT
• Kim IJ

• KMID: 2174998
• DOI: http://doi.org/10.4048/jbc.2007.10.1.29

Abstract

PURPOSE: Numerous non-invasive imaging methods for evaluating the chemotherapy response of breast cancer patients are currently being explored. The aim of present study was to investigate whether the washout rates (WRs) of 99mTc-MIBI could predict the response to chemotherapy in patients suffering with infiltrating ductal carcinoma using the expressions of multidrug resistance-related protein (MRP) and P-glycoprotein (Pgp).
METHODS
From May 2002 and March 2004, the patients were randomly and consecutively selected according to the results of immunohistochemical analyses of breast carcinoma specimens before the administration of neoadjuvant chemotherapy. A total 45 infiltrating ductal carcinomas in 45 female patients were selected and they were separated into three groups: group A consisted of tumors with both negative Pgp and MRP expressions (n=15); group B consisted of the tumors that were positive for either a Pgp expression or a MRP expression (n=15); group C consisted of the tumors that were positive for both Pgp and MRP expressions (n=15). All the patients were referred for double phase 99mTc-MIBI mammoscintigraphy after the injection of 925 MBq of 99mTc-MIBI to calculate the WR. The tumor response was evaluated after completion of neoadjuvant chemotherapy. The tumor response was classified as a complete or partial response (the responder group) and stable or progression (the non-responder group). All the patients underwent surgery.
RESULTS
The response rate of group C was lower than that of the other groups, but the difference was not statistically significant (p=0.283). The WR of non-responder group was lower than that of the responder group, although the difference was not statistically significant (p=0.674). The washout rates of group C was the highest than other groups and the difference was statistically significant (p=0.001).
CONCLUSION
In conclusion, the WR of 99mTc-MIBI is helpful for in vivo determination of both the Pgp and MRP expressions for infiltrating ductal carcinoma of the breast.

Keyword

Multidrug resistance-related protein; P-glycoprotein; 99mTc-MIBI Washout rates

MeSH Terms

Breast Neoplasms
Breast*
Carcinoma, Ductal*
Drug Therapy*
Female
Humans
P-Glycoprotein*
P-Glycoprotein

Figure

• 

  Fig 1 Immunohistochemical staining of Pgp and MRP in infiltrating ductal carcinomas. Examples of Pgp staining (A, non-expression; B, expression) and MRP staining (C, non-expression; D, expression) (×400).

• 

  Fig 2 Double phase 99mTc-MIBI mammoscintigraphy (early and delayed images) Region of interest (ROI) (C) were applied on the lesion and contralateral normal breast to calculate L/B ratio and washout rates (%) of early and delayed images (A, B). (A 34-yr-old woman with infiltrating ductal carcinoma in right breast).

• 

  Fig 3 Washout rates of 99mTc-MIBI according to the response to neoadjuvant chemotherapy. The Washout rates of non-responder group was lower than that of the responder group, although the difference was not statistically significant (p=0.674).

• 

  Fig 4 Washout rates of 99mTc-MIBI according to Pgp and MRP expression groups. The washout rates of group C was the highest than other groups and the difference was statistically significant (p=0.001).

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