Ewha Med J.  1991 Dec;14(4):389-395. 10.12771/emj.1991.14.4.389.

Effect of Various Irrigation Fluids of Arterial and Venous Endothelium after Ischemia

• Yoon CH
• Kim HJ

• KMID: 2170869
• DOI: http://doi.org/10.12771/emj.1991.14.4.389

Abstract

This study examines the extent of endothelial damage following a period of irrigation with various crystalloid irrigation fluds. Both arteries and veins were evaluated after irrigation with normal saline, lactated Ringer's. balanced salt solution (BSS), and balanced salt solution plus(BSS+). The arterial and venous endothelia were examined with the scanning electron microscope. Using a randomized blind observer scoring system. micrographs were evaluated for changes in nuclear shape, cell junction integrity, cytoplasm changes, and sloughing of the endothelial cell layer. BSS+ and BSS produced stastically significant(p<.001) improvement over the other two irrigation fluids in the arteries. BSS+ and BSS were stastically superior(P<.00l) in the venous vessels. The compositions of BSS and BSS+ tend to maintain a physiologic environment in the presence of ischemia. These maintained a morphologic appearance closer to that of perfusion-fixed controls The data suggest a protective effect of such physiologic preparations on the endothelium. The preservation of intact endothelium may play a role in decreased platelet activation. continued production of prostacyclin, and maintenance of and intact barrier between the intra-cellular and extracellular spaces. This could enhance the survival of transplanted or transferred tissue, by helping to maintain nearly normal endothelium during surgery.

MeSH Terms

Arteries
Cell Nucleus Shape
Cytoplasm
Endothelial Cells
Endothelium*
Epoprostenol
Extracellular Space
Ischemia*
Platelet Activation
Veins
Epoprostenol