J Audiol Otol.  2015 Dec;19(3):172-177. 10.7874/jao.2015.19.3.172.

Analysis of Bacterial Biofilms on a Cochlear Implant Following Methicillin-Resistant Staphylococcus Aureus Infection

• Im GJ
• An YS
• Choi J
• Song JJ
• Chae SW
• Jung HH

• KMID: 2167530
• DOI: http://doi.org/10.7874/jao.2015.19.3.172

Abstract

To demonstrate biofilm formations on a cochlear implant magnet of a pediatric patient suffering from a methicillin-resistant Staphylococcus aureus (MRSA) infection. The appearance of biofilm colonies was analyzed on different magnet sections. The appearance of MRSA biofilms on the surface of an explanted cochlear implant was analyzed by scanning electron microscopy (SEM), focusing on the pattern of extracellular polymeric substances (EPS) within the biofilms. SEM revealed unique biofilms with a three-dimensional EPS complex and tower-like formations. Biofilm configurations changed from the margin to the center of the magnet. Biofilms were solitary and scattered at the margin; large and plate-like in the center; and stacked in layers, forming towers and water channels, in the middle region. After a MRSA infection, biofilm formations were observed on the surface of a magnet. Bacterial biofilms provide optimal conditions for bacterial growth and antibiotic resistance and can cause intractable infections that lead to device failure.

Keyword

Biofilm; Scanning electron microscopy; Extracellular polymeric substance; Cochlear implant; Methicillin-resistant Staphylococcus aureus

MeSH Terms

Aquaporins
Biofilms*
Cochlear Implants*
Drug Resistance, Microbial
Equipment Failure
Humans
Methicillin Resistance*
Methicillin-Resistant Staphylococcus aureus*
Microscopy, Electron, Scanning
Polymers
Aquaporins
Polymers