Clin Exp Reprod Med.  2015 Dec;42(4):175-180. 10.5653/cerm.2015.42.4.175.

The effect of embryo catheter loading technique on the live birth rate

• Omidi M
• Halvaei I
• Mangoli E
• Khalili MA
• Razi MH

• KMID: 2167482
• DOI: http://doi.org/10.5653/cerm.2015.42.4.175

Abstract

OBJECTIVE
Embryo loading (EL) is a major step in embryo transfer (ET) and affect on the success of in vitro fertilization (IVF). This study aimed to compare the effect of two different EL techniques on the rates of pregnancy and delivery in IVF/ET cycles.
METHODS
207 fresh ET and 194 Frozen-thawed ET (FET) cycles were included in this retrospective study. Two groups (A and B) were defined based on the EL technique used. In group A, the entire catheter was flushed with Ham's F-10 medium. The embryos were then drawn into the catheter using one air bracket. In group B, 70 microL of air was aspirated into the syringe and the catheter was flushed using Ham's F10 medium. The medium, air, embryos, air, and finally another layer of medium were then sequentially drawn into the catheter. The main outcome measures were the pregnancy and delivery rates.
RESULTS
The groups did not differ with respect to the etiology of infertility, the source of spermatozoa, the quality of the embryos, the type of EL catheter, and the ease of transfer. The pregnancy rate was similar between two groups. In fresh ET cycles, a higher delivery rate was observed in group B than it group A (78.1% vs. 60%, p=0.1). In FET cycles, the rate of delivery was significantly higher in group B than in group A to a nonsignificant extent (88.9% vs. 58.8%, p=0.06).
CONCLUSION
EL techniques did not have a significant impact on the delivery rate in either fresh or FET cycles.

Keyword

Embryo loading technique; Embryo transfer; Pregnancy

MeSH Terms

Catheters*
Embryo Transfer
Embryonic Structures*
Fertilization in Vitro
Infertility
Live Birth*
Outcome Assessment (Health Care)
Pregnancy
Pregnancy Rate
Retrospective Studies
Spermatozoa
Syringes