Chromosomal Information of 1,144 Korean BAC Clones

Park, Mi Hyun; Lee, Hee Jung; Kim, Kwang Joong; Jeon, Jae Pil; Lee, Hye Ja; Kim, Jun Woo; Kim, Hung Tae; Cha, Hyo Soung; Kim, Cheol Hwan; Choi, Kang Yell; Park, Chan; Oh, Bermseok; Kimm, Kuchan; Lee, Jong Young; Han, Bok Ghee

Abstract

We sequenced 1,841 BAC clones by terminal sequencing, and 1,830 of these clones were characterized with regard to their human chromosomal location and gene content using Korean BAC library constructed at the Korean Science (KCGS). Sequence analyses of the 1,830 BAC clones was performed for chromosomal assignment: 1,144 clones were assigned to a single chromosome, 190 clones apparently assigned to more than one chromosome, and 496 clones to no chromosome. Evaluating gene content of the 1,144 BAC clones, we found that 706 clones represented 1,069 genes of which 415 genes existed in the BAC clones covering the full sequence of the gene, 180 genes covering a 50%~99%, and 474 genes covering less than 50% of the gene coverage. The estimated covering size of the KBAC clones was 73,379 kilobases (kb), in total corresponding to 2.3% of haploid human genome sequence. The identified BAC clones will be a public genomic resource for mapped clones for diagnostic and functional studies by Korean scientists and investigators worldwide.