Korean J Parasitol.  2016 Apr;54(2):173-179. 10.3347/kjp.2016.54.2.173.

Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)

• Liu ZX
• Zhang Y
• Liu YT
• Chang QC
• Su X
• Fu X
• Yue DM
• Gao Y
• Wang CR

• KMID: 2164014
• DOI: http://doi.org/10.3347/kjp.2016.54.2.173

Abstract

Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

Keyword

Echinostoma hortense; complete mitochondrial DNA; echinostomatidae; phylogenetic analysis

MeSH Terms

Amino Acid Sequence
Base Sequence
Databases, Nucleic Acid
Dataset
Echinostoma*
Echinostomatidae
Fasciolidae
Genes, rRNA
Genetic Markers
Genetics, Population
Genome
Genome, Mitochondrial*
Humans
RNA, Transfer
Trematoda
Genetic Markers
RNA, Transfer