J Lab Med Qual Assur.  2016 Mar;38(1):52-57. 10.15263/jlmqa.2016.38.1.52.

Performance Evaluation of the VISTA 500

• Ko DH
• Cho EJ
• Lee W
• Chun S
• Min WK

• KMID: 2162122
• DOI: http://doi.org/10.15263/jlmqa.2016.38.1.52

Abstract

In this study, we evaluated the performance of a recently developed immunoassay analyser, the VISTA 500 (Siemens, Germany). Precision, linearity, and comparison studies were performed according to the Clinical and Laboratory Standards Institute guidelines. The test items evaluated included IgG, IgA, IgM, C3, C4, ceruloplasmin, prealbumin, transferrin, haptoglobin, rheumatoid factor, anti-streptolysin O, and cystatin C. Commercial control materials (BioRad Laboratories, USA), commercial linearity validation materials (Maine Standards, USA), and patient samples were used for the evaluation. For the correlation study, analysis with a BN-II nephelometer (Siemens) was used as a comparative method. Total coefficients of variation of analytes were found to be between 1.9% and 5.5%. Results of the linearity evaluation were also acceptable for the range tested. Correlations with comparative methods were acceptable. The VISTA 500 analyser showed satisfactory analytical performance with respect to precision, linearity, and comparison. We conclude that the VISTA 500 is likely a good candidate as an immunology analyser.

Keyword

VISTA 500; Performance; Evaluation studies as topic

MeSH Terms

Allergy and Immunology
Ceruloplasmin
Cystatin C
Evaluation Studies as Topic
Haptoglobins
Humans
Immunoassay
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Prealbumin
Rheumatoid Factor
Statistics as Topic
Transferrin
Ceruloplasmin
Cystatin C
Haptoglobins
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Prealbumin
Rheumatoid Factor
Transferrin