Clin Exp Reprod Med.  2016 Mar;43(1):31-37. 10.5653/cerm.2016.43.1.31.

The effects of blastocyst morphological score and blastocoele re-expansion speed after warming on pregnancy outcomes

Affiliations
  • 1Reproductive Medicine Center, 105th Hospital of PLA, Hefei, China. jiangh105@sina.com

Abstract


OBJECTIVE
The aim of this study was to investigate associations between the morphology score of blastocysts and blastocoele re-expansion speed after warming with clinical outcomes, which could assist in making correct and cost-effective decisions regarding the appropriate time to vitrify blastocysts and to transfer vitrified-warmed blastocysts.
METHODS
A total of 327 vitrified-warmed two-blastocyst transfer cycles in women 38 years old and younger were included in this retrospective study.
RESULTS
The clinical pregnancy rate (CPR) and implantation rate (IR) of transfers of two good-morphology grade 4 blastocysts vitrified on day 5 (64.1% and 46.8%, respectively) were significantly higher than the CPR and IR associated with the transfers of two good-morphology grade 3 blastocysts vitrified on day 5 (46.7% and 32.2%, respectively). No significant differences were found in the CPR and IR among the transfers of two good-morphology grade 4 blastocysts regardless of the day of cryopreservation. Logistic regression analysis showed that blastocoele re-expansion speed after warming was associated with the CPR.
CONCLUSION
The selection of a good-morphology grade 4 blastocyst to be vitrified could be superior to the choice of a grade 3 blastocyst. Extending the culture of grade 3 blastocysts and freezing grade 4 or higher blastocysts on day 6 could lead to a greater likelihood of pregnancy. Since re-expansion was shown to be a morphological marker of superior blastocyst viability, blastocysts that quickly re-expand after warming should be prioritized for transfer.

Keyword

Blastocyst; Embryo transfer; Embryonic development; Freezing; Pregnancy outcome

MeSH Terms

Blastocyst*
Cardiopulmonary Resuscitation
Cryopreservation
Embryo Transfer
Embryonic Development
Female
Freezing
Humans
Logistic Models
Pregnancy
Pregnancy Outcome*
Pregnancy Rate
Pregnancy*
Retrospective Studies
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