Exp Mol Med.
2015 Mar;47(3):e152. 10.1038/emm.2014.115.
Euodia sutchuenensis Dode extract stimulates osteoblast differentiation via Wnt/beta-catenin pathway activation
- Affiliations
-
- 1Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea. kychoi@yonsei.ac.kr
- 2Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
- 3Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
- 4Department of Molecular Biology, College of Natural Science, Pusan National University, Pusan, Korea.
- KMID: 2155417
- DOI: http://doi.org/10.1038/emm.2014.115
Abstract
- The Wnt/beta-catenin pathway has a role in osteoblast differentiation and bone formation. We screened 100 plant extracts and identified an extract from Euodia sutchuenensis Dode (ESD) leaf and young branch as an effective activator of the Wnt/beta-catenin pathway. ESD extract increased beta-catenin levels and beta-catenin nuclear accumulation in murine primary osteoblasts. The ESD extract also increased mRNA levels of osteoblast markers, including RUNX2, BMP2 and COL1A1, and enhanced alkaline phosphatase (ALP) activity in murine primary osteoblasts. Both ESD extract-induced beta-catenin increment and ALP activation were abolished by beta-catenin knockdown, confirming that the Wnt/beta-catenin pathway functions in osteoblast differentiation. ESD extract enhanced terminal osteoblast differentiation as shown by staining with Alizarin Red S and significantly increased murine calvarial bone thickness. This study shows that ESD extract stimulates osteoblast differentiation via the Wnt/beta-catenin pathway and enhances murine calvarial bone formation ex vivo.
MeSH Terms
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Animals
Cell Differentiation/*drug effects
Evodia/*chemistry
HEK293 Cells
Humans
Mice
Osteoblasts/cytology/*drug effects/*metabolism
Osteogenesis/drug effects
Plant Extracts/chemistry/*pharmacology
Skull/anatomy & histology/drug effects/metabolism
Wnt Signaling Pathway/*drug effects
beta Catenin/genetics/metabolism
Plant Extracts
beta Catenin
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