J Korean Med Sci.  2014 Nov;29(Suppl 3):S193-S200. 10.3346/jkms.2014.29.S3.S193.

Effects of Platelet-Rich Plasma, Adipose-Derived Stem Cells, and Stromal Vascular Fraction on the Survival of Human Transplanted Adipose Tissue

• Kim DY
• Ji YH
• Kim DW
• Dhong ES
• Yoon ES

• KMID: 2151413
• DOI: http://doi.org/10.3346/jkms.2014.29.S3.S193

Abstract

Traditional adipose tissue transplantation has unpredictable viability and poor absorption rates. Recent studies have reported that treatment with platelet-rich plasma (PRP), adipose-derived stem cells (ASCs), and stromal vascular fraction (SVF) are related to increased survival of grafted adipose tissue. This study was the first simultaneous comparison of graft survival in combination with PRP, ASCs, and SVF. Adipose tissues were mixed with each other, injected subcutaneously into the back of nude mice, and evaluated at 4, 8, and 12 weeks. Human adipocytes were grossly maintained in the ASCs and SVF mixtures. Survival of the adipose tissues with PRP was observed at 4 weeks and with SVF at 8 and 12 weeks. At 12 weeks, volume reduction in the ASCs and SVF mixtures were 36.9% and 32.1%, respectively, which were significantly different from that of the control group without adjuvant treatment, 51.0%. Neovascular structures were rarely observed in any of the groups. Our results suggest that the technique of adding ASCs or SVF to transplanted adipose tissue might be more effective than the conventional grafting method. An autologous adipose tissue graft in combination with ASCs or SVF may potentially contribute to stabilization of engraftment.

Keyword

Adipose Tissue; Tissue Graft; Platelet-Rich Plasma; Stem Cell

MeSH Terms

Adipocytes/*transplantation
Adipose Tissue/cytology/*transplantation
Adult
Animals
Female
*Graft Survival
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
*Platelet-Rich Plasma
Stem Cells
Stromal Cells/*transplantation
Transplantation, Heterologous

Figure

• 

  Fig. 1 Schematic of the study procedures. The mouse's back was divided into four quadrants. Fresh human adipose tissues with ASCs, SVF, and PRP were injected subcutaneously. PBS, phosphate-buffered saline; ASCs, adipose-derived stem cells; SVF, stromal vascular fraction; PRP, platelet-rich plasma.

• 

  Fig. 2 Surviving volume of grafted human fat tissue. (A) The macroscopic appearance showed that neovasculature structures were observed in the adjuvant groups after 8 weeks. (B) Volumes of the surviving adipose tissues. For the ASCs and SVF groups, the mean volumes were significantly greater than that of the control at 12 weeks. Mean and 95% CI are shown (*P < 0.05). ASCs, adipose-derived stem cells; SVF, stromal vascular fraction; PRP, platelet-rich plasma.

• 

  Fig. 3 Representative images from hematoxylin and eosin staining. In the ASCs and SVF groups, the structure of the fat cells was generally maintained at 8 weeks after transplantation. At 12 weeks, the cells in the SVF group showed a more stable form than in any of the other groups (100×). ASCs, adipose-derived stem cells; SVF, stromal vascular fraction; PRP, platelet-rich plasma.

• 

  Fig. 4 Representative images of CD31 antibody staining. Neovasculature structures were observed on the side with adjuvants at all time points. However, vascular endothelial cells were scarcely stained by CD31 antibodies with human reactivity (200×). ASCs, adipose-derived stem cells; SVF, stromal vascular fraction; PRP, platelet-rich plasma.

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