Immune Netw.  2012 Dec;12(6):291-295. 10.4110/in.2012.12.6.291.

The Role of the Ethylacetate Fraction from Hydnocarpi Semen in Acute Inflammation In Vitro Model

• Lee GS
• Shim H
• Lee KM
• Kim SH
• Yim D
• Cheong JH
• Kang TJ

• KMID: 2150762
• DOI: http://doi.org/10.4110/in.2012.12.6.291

Abstract

We previously reported that Hydnocarpi Semen (HS) has a wound healing effect on diabetic foot ulcer lesion in mice. In this study, ethylacetate (EtOAc) fraction from HS extract were evaluated for their wound healing activity by using in vitro acute inflammation model. GC and GC/MS analysis shows that the main constituents in EtOAc fraction are chaulmoogric acid, hydnocarpic acid, and gorlic acid. EtOAc fraction activated macrophages to increase the production of TNF-alpha. The fraction also increased the production of TGF-beta and VEGF, which induced fibroblast activation and angiogenesis. These results suggest that the mechanism that the fraction helps to enhance healing of skin wound is possibly associated with the production of TNF-alpha, as well as secretion of VEGF, TGF-beta and HS may have a new bioactive material for the treatment of skin wound.

Keyword

Hydnocarpi Semen; Ethylacetate fraction; Inflammation; Cytokines

MeSH Terms

Animals
Cytokines
Diabetic Foot
Fatty Acids
Fibroblasts
Inflammation
Macrophages
Mice
Semen
Skin
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Ulcer
Vascular Endothelial Growth Factor A
Wound Healing
Cytokines
Fatty Acids
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A

Figure

• 

  Figure 1 The scheme for the preparation of EtOAc fraction from Hydnocarpi Semen. This figure was adapted from a scheme in reference 7.

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  Figure 2 Typical chromatogram of fatty acids in EtOAc fraction from crude extract of Hydnocarpi Semen by GC. Each peak indicates (a) Chaulmoogric acid, (b) Hydnocarpic acid, (c) Gorlic acid.

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  Figure 3 The effect of EtOAc fraction on cytokine production in RAW 264.7 cell. The cells were treated with the fraction at a dose dependent manner for 24, and cell culture supernatants were collected and used for (A) TNF-α, (B) TGF-β, and (C) VEGF assay. Data are representative of at least three independent experiments, each done in triplicate; *p <0.05, **p<0.01 compared to non-treated cells.

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