Korean J Clin Pathol.  1997 Dec;17(6):1150-1156.

The Restraint of Umbilical Cord Blood Volume for Transplantation

• No authors listed

Abstract

BACKGROUND: Umbilical cord blood (UCB) has been suggested as an alternate source of stem cells in bone marrow transplantation because of lower incidence of graft versus host disease (GVHD) and ease of collection. However, limited volume of UCB makes it difficult to collect sufficient amount of stem cells not only for adults but also even for children. Therefore, we tried to evaluate the amount of stem cells collected using UCB as a source.
METHODS
The collection of UCB was performed in 40 full-term deliveries which consisted of 16 vaginal deliveries and 24 Cesarean sections. The collection was made by gravity and uterine contraction before placental expulsion with 320 mL-blood bag containing CPDA-1 anticoagulant, and by gravity and milking after placental expulsion with 50 mL-comical tube containing 10 unit/mL heparin or CPDA-15 mL.
RESULTS
Mean volume of collected UCB was 108 mL (57-192 mL). The average rate of cell viability resealed 98.1%. The average number of nucleated, mononucleated, and CD34 positive cells per mL of blood were 1.10x10(7)/m1, 4.23x 10(6)/mL, and 1.07x10(5)/mL, respectively. The average number of total nucleated cells and mononucleated cells were 1.22x10(9) and 4.62x 10(8), respectively. The average number of total CD34 positive cells was 12.2x10(6). If the transplantation of 5x10(7) nucleated cells per kg body weight or 0.5x10(6) CD34 positive cells per kg body weight is required for engraftment, the average body weight of recipient is 24.4 kg (8.8-50.0 kg for total nucleated cells, 3.5-60.6 kg for CD34 positive cells).
CONCLUSIONS
By our results, it is possible to use UCB to transplant children under 8 years-old age or approximately 25 kg body weight. The way to overcome this volume limitation will be ex vivo expansion of UCB progenitor cells.

MeSH Terms

Adult
Body Weight
Bone Marrow Transplantation
Cell Survival
Cesarean Section
Child
Female
Fetal Blood*
Graft vs Host Disease
Gravitation
Heparin
Humans
Incidence
Milk
Pregnancy
Stem Cells
Umbilical Cord*
Uterine Contraction
Heparin