Korean J Gynecol Oncol.  2005 Dec;16(4):347-353.

Effects of cell growth inhibition on the combination of cisplatin with green tea extracts

• Kim HK
• Kang YH
• Kwak SY
• Ding GH
• Bae SM
• Park EK
• Lee YS
• Kim J
• Kim YW
• Ro DY
• Lee JM
• Namkoong S
• Chang HS
• Chun HJ
• Lim DS
• Ahn WS

Abstract

OBJECTIVE
The chemotherapeutic agent cisplatin (cis-diamminedichloroplatinum (II)) is particularly effective against cervical cancer. The purpose of this study is to elucidate combination effect of cisplatin and green tea extracts on the growth inhibition of TC-1 cell.
METHODS
To observe the anti-proliferative effects, we treated different doses of cisplatin (0.1, 0.5, 2.5 uM), GTP (1, 5, 25 ug/ml) and EGCG (25, 50, 100 uM). to TC-1 cells. Also, we treated 0.5 uM of cisplatin and different doses of GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM). Cell viability was scored by use of MTT assay. In addition, E6 gene expression patterns in TC-1 cell were investigated by using RT-PCR.
RESULTS
Cell growth inhibition in a dose dependent was observed at the different concentration of ciaplatin, GTP and EGCG. Also, in the groups treated by 0.5 uM of cisplatin and GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM), the inhibition of cell growth showed with 12.2%, 6.9% and 63.4%, 72.2% as compared to the group treated by cisplatin only. In RT-PCR, down regulation of E6 was shown.
CONCLUSION
Additive effect of the combination of cisplatin with GTP or EGCG on the inhibition of cell growth was observed. This effect suggests the possibility lowering the concentration of chemotherapeutic drugs, which alleviate the side effect of drugs.

Keyword

Cisplatin; TC-1 cell line; GTP; EGCG

MeSH Terms

Cell Survival
Cisplatin*
Down-Regulation
Gene Expression
Guanosine Triphosphate
Tea*
Uterine Cervical Neoplasms
Cisplatin
Guanosine Triphosphate
Tea