Arch Plast Surg.  2013 Sep;40(5):517-521. 10.5999/aps.2013.40.5.517.

Expression of Extracellular Superoxide Dismutase Protein in Diabetes

• Kim CH

• KMID: 2120806
• DOI: http://doi.org/10.5999/aps.2013.40.5.517

Abstract

BACKGROUND
Diabetes is characterized by chronic hyperglycemia, which can increase reactive oxygen species (ROS) production by the mitochondrial electron transport chain. The formation of ROS induces oxidative stress and activates oxidative damage-inducing genes in cells. No research has been published on oxidative damage-related extracellular superoxide dismutase (EC-SOD) protein levels in human diabetic skin. We investigated the expression of EC-SOD in diabetic skin compared with normal skin tissue in vivo.
METHODS
The expression of EC-SOD protein was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was also carried out to confirm the EC-SOD expression level in the 6 diabetic skin tissue samples.
RESULTS
The western blotting showed significantly lower EC-SOD protein expression in the diabetic skin tissue than in the normal tissue. Immunohistochemical examination of EC-SOD protein expression supported the western blotting analysis.
CONCLUSIONS
Diabetic skin tissues express a relatively small amount of EC-SOD protein and may not be protected against oxidative stress. We believe that EC-SOD is related to the altered metabolic state in diabetic skin, which elevates ROS production.

Keyword

Skin; Diabetes mellitus; Superoxide dismutase

MeSH Terms

Blotting, Western
Diabetes Mellitus
Electron Transport
Humans
Hyperglycemia
Oxidative Stress
Reactive Oxygen Species
Skin
Superoxide Dismutase
Superoxides
Reactive Oxygen Species
Superoxide Dismutase
Superoxides