Effect Of Polyphosphate On Skin Wound Healing

Park, Gi Bum; Ryu, Dong Mok; Lee, Jin Yong; Shin, Je Won

Abstract

Polyphosphate has been used as a food additive.It has been approved the use of PolyP to the products as a safe additive.In other study,PolyP seems to has an ability of promoting effect on bone regeneration,possibly by enhancing osteoconductivity of the carrier and/or by increasing osteoinductivity of the damaged bone tissue. So we performed this study in order to evaluate the effect of polyphosphate on the dermal defect healing in critical-sized defect wound.Using welder and blade,a 1cm diameter sized burn-defect and rectangular excisional-defect was prepared in the thigh of each Sprague-Dawley rats. Then defect areas were applied topically with distilled water(DW), 0.01%orthophosphate(Pi), or 0.01%polyP75. The application of these materials was continued for seven days. The rats, which had burn wounds,were sacrificed at week 1 and 2.the other groups,which had excisional wounds, were sacrificed at day 3,week 1,2, and 4.the specimens of the defect were prepared,stained with Hand E, and examined under the microscope. Dermal defect healing capacity of polyP75 was also examined by observaing cellular activity of periodontal cells using MTT assay. The results were as follows. PolyP75 at the concentrations of 0.01%increased the MTT activity of periodontal cells by 154.1%.a pathohistologic findings of burn wound with polyP75 showed a faster epidermal healing and more profound maturation in dermis than those of other groups. In excisional wound,compare with other gruops, tissue maturity and density of matrix in dermis were much enhanced in the polyP75 groups.