J Adv Prosthodont.  2015 Apr;7(2):93-97. 10.4047/jap.2015.7.2.93.

The effect of two artificial salivas on the adhesion of Candida albicans to heat-polymerized acrylic resin

Affiliations
  • 1Department of Prosthodontics, Tepebasi Oral and Dental Health Hospital, Ankara, Turkey.
  • 2Department of Prosthodontics, Faculty of Dentistry, Gazi University, Ankara, Turkey. dtduygukc@hotmail.com
  • 3Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.

Abstract

PURPOSE
Xerostomia can diminish the quality of life, leads to changes in normal chemical composition of saliva and oral microbiata, and increases the risk for opportunistic infections, such as Candida albicans. Various artificial salivas have been considered for patients with xerostomia. However, the knowledge on the antifungal and antiadhesive activity of artificial saliva substitutes is limited. The aim of the present study was to evaluate influence of two artificial salivas on the adhesion of Candida albicans to the polymethylmethacrylate disc specimens.
MATERIALS AND METHODS
Two commercial artificial salivas (Saliva Orthana and Biotene Oral Balance Gel) were selected. 45 polymethylmethacrylate disc specimens were prepared and randomly allocated into 3 groups; Saliva Orthana, Biotene-Oral Balance gel and distilled water. Specimens were stored in the artificial saliva or in the sterile distilled water for 60 minutes at 37degrees C. Then they were exposed to yeast suspensions including Candida albicans. Yeast cells were counted using x40 magnification under a light microscope and data were analysed.
RESULTS
Analysis of data indicated statistically significant difference in adhesion of Candida albicans among all experimental groups (P=.000). Findings indicated that Saliva Orthana had higher adhesion scores than the Biotene Oral Balance gel and distilled water (P<.05).
CONCLUSION
In comparison of Saliva Orthana, the use of Biotene Oral Balance Gel including lysozyme, lactoferrin and peroxidase may be an appropriate treatment method to prevent of adhesion of Candida albicans and related infections in patients with xerostomia.

Keyword

Artificial Saliva; Candida albicans; Xerostomia

MeSH Terms

Candida albicans*
Humans
Lactoferrin
Muramidase
Opportunistic Infections
Peroxidase
Polymethyl Methacrylate
Quality of Life
Saliva
Saliva, Artificial*
Suspensions
Water
Xerostomia
Yeasts
Lactoferrin
Muramidase
Peroxidase
Polymethyl Methacrylate
Saliva, Artificial
Suspensions
Water

Reference

1. Al-Dwairi Z, Lynch E. Xerostomia in complete denture wearers: prevalence, clinical findings and impact on oral functions. Gerodontology. 2014; 31:49–55.
2. Dodds MW, Johnson DA, Yeh CK. Health benefits of saliva: a review. J Dent. 2005; 33:223–233.
3. Hatti S, Ravindra S, Satpathy A, Kulkarni RD, Parande MV. Biofilm inhibition and antimicrobial activity of a dentifrice containing salivary substitutes. Int J Dent Hyg. 2007; 5:218–224.
4. Tenovuo J. Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: efficacy and safety. Oral Dis. 2002; 8:23–29.
5. Arslan A, Orhan K, Canpolat C, Delilbasi C, Dural S. Impact of xerostomia on oral complaints in a group of elderly Turkish removable denture wearers. Arch Gerontol Geriatr. 2009; 49:263–267.
6. Torres SR, Peixoto CB, Caldas DM, Silva EB, Akiti T, Nucci M, de Uzeda M. Relationship between salivary flow rates and Candida counts in subjects with xerostomia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 93:149–154.
7. Silva MP, Chibebe Junior J, Jorjão AL, Machado AK, Oliveira LD, Junqueira JC, Jorge AO. Influence of artificial saliva in biofilm formation of Candida albicans in vitro. Braz Oral Res. 2012; 26:24–28.
8. Hahnel S, Ettl T, Gosau M, Rosentritt M, Handel G, Bürgers R. Influence of saliva substitute films on the initial adhesion of Candida albicans to dental substrata prior to and after artificial ageing. Arch Oral Biol. 2010; 55:391–396.
9. Shahdad SA, Taylor C, Barclay SC, Steen IN, Preshaw PM. A double-blind, crossover study of Biotène Oralbalance and BioXtra systems as salivary substitutes in patients with postradiotherapy xerostomia. Eur J Cancer Care (Engl). 2005; 14:319–326.
10. Hahnel S, Behr M, Handel G, Bürgers R. Saliva substitutes for the treatment of radiation-induced xerostomia-a review. Support Care Cancer. 2009; 17:1331–1343.
11. Epstein JB, Emerton S, Le ND, Stevenson-Moore P. A double-blind crossover trial of Oral Balance gel and Biotene toothpaste versus placebo in patients with xerostomia following radiation therapy. Oral Oncol. 1999; 35:132–137.
12. Güneri P, Alpöz E, Epstein JB, Çankaya H, Ateş M. In vitro antimicrobial effects of commercially available mouth-wetting agents. Spec Care Dentist. 2011; 31:123–128.
13. Meyer-Lueckel H, Schulte-Mönting J, Kielbassa AM. The effect of commercially available saliva substitutes on predemineralized bovine dentin in vitro. Oral Dis. 2002; 8:192–198.
14. Young B, Jose A, Cameron D, McCord F, Murray C, Bagg J, Ramage G. Attachment of Candida albicans to denture base acrylic resin processed by three different methods. Int J Prosthodont. 2009; 22:488–489.
15. Radford DR, Challacombe SJ, Walter JD. Denture plaque and adherence of Candida albicans to denture-base materials in vivo and in vitro. Crit Rev Oral Biol Med. 1999; 10:99–116.
16. Zamperini CA, Machado AL, Vergani CE, Pavarina AC, Rangel EC, Cruz NC. Evaluation of fungal adherence to plasmamodified polymethylmethacrylate. Mycoses. 2011; 54:e344–e351.
17. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent. 1998; 26:577–583.
18. Henriques M, Azeredo J, Oliveira R. Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite. Colloids and Surfaces B. Biointerface. 2004; 33:235–241.
19. Yildirim MS, Hasanreisoglu U, Hasirci N, Sultan N. Adherence of Candida albicans to glow-discharge modified acrylic denture base polymers. J Oral Rehabil. 2005; 32:518–525.
20. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol. 1995; 22:1–14.
21. San Millán R, Elguezabal N, Regúlez P, Moragues MD, Quindós G, Pontón J. Effect of salivary secretory IgA on the adhesion of Candida albicans to polystyrene. Microbiology. 2000; 146:2105–2112.
22. Edgerton M, Scannapieco FA, Reddy MS, Levine MJ. Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Infect Immun. 1993; 61:2644–2652.
23. Anil S, Samaranayake LP. Impact of lysozyme and lactoferrin on oral Candida isolates exposed to polyene antimycotics and fluconazole. Oral Dis. 2002; 8:199–206.
24. Tenovuo J. Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: efficacy and safety. Oral Dis. 2002; 8:23–29.
25. Kho HS, Kim YY, Chang JY, Kim MJ, Lee SG. Candidacidal activities of the glucose oxidase-mediated lactoperoxidase system. Arch Oral Biol. 2012; 57:684–688.
26. Nikawa H, Hamada T. Binding of salivary or serum proteins to Candida albicans in vitro. Arch Oral Biol. 1990; 35:571–573.
27. Flisfisch S, Meyer J, Meurman JH, Waltimo T. Effects of fluorides on Candida albicans. Oral Dis. 2008; 14:296–301.
28. Sugiura Y, Soga Y, Tanimoto I, Kokeguchi S, Nishide S, Kono K, Takahashi K, Fujii N, Ishimaru F, Tanimoto M, Yamabe K, Tsutani S, Nishimura F, Takashiba S. Antimicrobial effects of the saliva substitute, Oralbalance, against microorganisms from oral mucosa in the hematopoietic cell transplantation period. Support Care Cancer. 2008; 16:421–424.
Full Text Links
  • JAP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr