J Korean Ophthalmol Soc.  1999 Aug;40(8):2087-2093.

Expression of Vascular Endothelial Growth Factor and iNOS in Pterygia

• Lee KJ
• Joo MJ
• Lee DH

Abstract

To evaluate the expression of vascular endothelial growth factor (VEGF) in pterygia and seek the reciprocal relationships between VEGF and nitric oxide (NO) in development of pterygia. Conjunctiva sampled during conjunc-tival transplantation of pterygial operation and pterygia were used in this study. OCT compound-fixed-cryopreserved tissues consisted normal conjunctiva and pterygia were used to study the expression of VEGF and iNOS with immunohistochemistry. For confirmation of NOS activity, NADPH-diaphorase staining was done. ELISA for detection of VEGF amounts was performed. The expression of VEGF and iNOS were revealed in the epithelium of pterygia, although there were not expressed in the epithelium of normal conjunctiva. The epithelium of pterygia was stained with NADPH-diaphorase. The results of ELISA showed the higher amount of VEGF inpterygia compared with conjunctiva. These findings suggest that VEGF and NO play an important role in fibrovascular development of pterygia.s

Keyword

Nitric oxide (NO); Pterygia; Vascular endothelial growth factor (VEGF)

MeSH Terms

Conjunctiva
Enzyme-Linked Immunosorbent Assay
Epithelium
Immunohistochemistry
Nitric Oxide
Vascular Endothelial Growth Factor A*
Nitric Oxide
Vascular Endothelial Growth Factor A