Korean J Parasitol.
2014 Dec;52(6):653-659. 10.3347/kjp.2014.52.6.653.
Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
- Affiliations
-
- 1Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.
- 2Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
- 3Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.
- 4Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
- 5Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. majid.fasihi@gmail.com
- KMID: 2069816
- DOI: http://doi.org/10.3347/kjp.2014.52.6.653
Abstract
- Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models.
MeSH Terms
-
Animals
Anthelmintics/isolation & purification/*pharmacology
Benzoquinones/isolation & purification/*pharmacology
Biological Assay
Echinococcosis/parasitology/veterinary
Echinococcus granulosus/*drug effects/isolation & purification
Gas Chromatography-Mass Spectrometry
Nigella sativa/*chemistry
Oils, Volatile/chemistry/isolation & purification/*pharmacology
Seeds/chemistry
Sheep
Sheep Diseases/parasitology
Survival Analysis
Time Factors
Anthelmintics
Benzoquinones
Oils, Volatile
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Spices Mycobiota and Mycotoxins Available in Saudi Arabia and Their Abilities to Inhibit Growth of Some Toxigenic Fungi
- Antidiabetic Activity of Nigella Sativa (Black Seeds) and Its Active Constituent (Thymoquinone) – A Review of Human and Experimental Animal Studies
- Scolicidal Effects of Olea europaea and Satureja khuzestanica Extracts on Protoscolices of Hydatid Cysts
- Chemopreventive and Anticancer Effects of Thymoquinone: Cellular and Molecular Targets
- Nigella Sativa-Coated Hydroxyapatite Scaffolds: Synergetic Cues to Stimulate Myoblasts Differentiation and Offset Infections
