J Korean Diabetes Assoc.  1999 Dec;23(6):768-776.

Differential Effects of Palmitate and Docosahexaenoic acid on ATP-sensitive K+ Channel Activity of Pancreatic beta-cells

• Kim YW
• Doh KO
• Song DK
• Bae JH
• Park WK
• Won KJ
• Lee HW
• Lee SK

Abstract

BACKGROUND: Elevated levels of free fatty acids markedly enhance insulin secretion. However, dietary polyunsaturated fatty supplementation decrease insulin secretion. The effects of different type of fatty acids on cultured pancreatic beta cell remain controversy. Therefore, the specific goal of this study was to confirm the effect of palmitate and docosahexaenoic acid (DHA) on pancreatic beta-cells. We measured ATP-sensitive K+ (KATP) channel activity by patch clamp technique. METHOD: Pancreatic beta-cells were isolated from male Sprague-Dawley rats and cultured on the cover glass in the culture media. KATP channel activity of pancreatic beta-cells were measured by the cell-attached mode of the patch clamp technique. We treated 30 micrometer of palmitate and DHA dissolved with 3% albumin solution. RESULT: 30 micrometer of palmitate inhibited KATP channel activity. Moreover, after additions of 5 and 10 mM glucose, additional and dose dependent inhibitory effects were revealed. However, 30 micrometer of DHA did not have these additional inhibitory effect treated with 5 and lOmM glucose. CONCLUSION: Palmitate as a saturated fatty acid inhibited activity of KATP channel and increased inhibitory effect of glucose on this channel activity, however, DHA as a polyunsaturated fatty acid attenuated inhibitory effect of glucose on this channel activity.

Keyword

ATP-sensitive K+ channels; Saturated fatty acid; Unsaturated fatty acid; Insulin secretion

MeSH Terms

Culture Media
Fatty Acids
Fatty Acids, Nonesterified
Glass
Glucose
Humans
Insulin
Insulin-Secreting Cells
Male
Rats, Sprague-Dawley
Culture Media
Fatty Acids
Fatty Acids, Nonesterified
Glucose
Insulin