J Korean Assoc Oral Maxillofac Surg.  2005 Feb;31(1):31-38.

Interpretation of bacterial contamination of allogeneic tissues obtained from cadaveric and living donors

• Lee EY

Abstract

Thorough screening of donors medical and social history, extensive serological and bacterial screening combined with developed processing and sterilization methods have improved the safety of the allogeneic tissues in recent decades. The risk of bacterial infection through allogenic tissue transplantation is one of the major problems facing tissue banks. The purpose study is to report the contamination rate in 358 retrieved tissues obtained strictly aseptic conditions, between 2001 and 2002 in Korea Tissue Bank. Samples from 9 donors(total 13 donors) were used in blood culture, and in 7 donors the blood culture were negative. Of the 358 tissues cultured in their entirety, 186(52%) were initially culture negative and 177(48%) were positive. Organism low pathogenicity were cultures from 20.2% of the tissues. To minimize the bacterial load, donors should be obtain in operating rooms, using aseptic techniques with only a few personnel for procurement. The procurement cultures from donors and retrieved tissues with multiple should be carefully interpreted. Blood cultures should be taken account, since these can help to find contamination not detect swab culture. A prospective cohort study is needed to determine which of the varied processing and sterilization methodologies gives the best quality.

Keyword

Allogeneic tissues; Blood culture; Cadaveric & living donors

MeSH Terms

Bacterial Infections
Bacterial Load
Cadaver*
Cohort Studies
Humans
Korea
Living Donors*
Mass Screening
Operating Rooms
Sterilization
Tissue Banks
Tissue Donors
Tissue Transplantation
Transplants
Virulence