J Bacteriol Virol.
2004 Mar;34(1):1-8.
groEL Gene Analysis of Borrelia afzelii Isolated in Korea
- Affiliations
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- 1Department of Microbiology, College of Medicine, Konkuk University, Chugju, Chungchungbuk-Do 380-701, Korea. shlee@kku.ac.kr
- 2Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799, Korea.
Abstract
- Eleven Borrelia afzelii strains, isolated from Ixodes nipponensis and Apodemus agrarius in Korea, were characterized by groEL gene analysis. Results from previous studies suggested that the groEL gene, which encodes the 60-kDa heat shock protein GroEL, was useful for the differentiation of B. burgdorferi sensu lato. The B. afzelii isolates could be divided into two groups by the phylogenetic tree constructed by UPGMA method and Tsp509 I PCR-RFLP analysis. The result suggested that the groEL gene is useful for identification and characterization of B. burgdorferi sensu lato though a short DNA fragment (310 bp) of the gene was sequenced and compared each other, and that Korean B. afzelii strains are heterogeneous genotypically.
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