Mycobiology.  2007 Sep;35(3):159-161. 10.4489/MYCO.2007.35.3.159.

Fungi Isolated from Pine Wood Nematode, Its Vector Japanese Pine Sawyer, and the Nematode-Infected Japanese Black Pine Wood in Korea

• Hyun MW
• Kim JH
• Suh DY
• Lee SK
• Kim SH

• KMID: 2052876
• DOI: http://doi.org/10.4489/MYCO.2007.35.3.159

Abstract

Pine tree death caused by pine wood nematode (PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle (JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. From JPS adult beetles, nine genera of Aspergillus, Gibberella, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidiomycetes were found. Ten genera from PWN-infected wood were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectrtia, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

Keyword

ITS rDNA; Japanese black pine; Japanese pine sawyer; Pine wood nematode; Pine wood nematode-associated fungi

MeSH Terms

Adult
Asian Continental Ancestry Group*
Aspergillus
Basidiomycota
Beetles
Botrytis
DNA, Ribosomal
Fungi*
Fusarium
Gibberella
Gyeongsangnam-do
Humans
Hypocrea
Korea*
Larva
Life Cycle Stages
Mucor
Ophiostoma
Penicillium
Pinus
Sequence Analysis
Trichoderma
Wood*
DNA, Ribosomal