Ewha Med J.  1996 Mar;19(1):119-124. 10.12771/emj.1996.19.1.119.

Fetal Cord Serum Supplemented Ham's F-10 vs. Medi-Cult for in Vitro Fertilization Culture Medium

Affiliations
  • 1Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Korea.

Abstract

The media for assisted fertilization from Medi-Cult is based on a Synthetic Serum Replacement(SSR). The main principle of SSR is a metal iron buffer containing a balanced mixture of iron and trace metals. Recently, Synthetic Serum Supplement(SSS) and Medi-cult are commonly used. These synthetic substitutes have some advandages, such as safty from viral contamination and no needs of bioassay. To compare the usefulness between the Medi-cult and Ham's F-10 supplemented with fetal cord serum for the culture medium of in vitro fertilization, fertilization rate, cleavage rate and pregnancy rate were studied. It is concluded that Medi-cult was better than Ham's F-10 supplemented with fetal cord serum according to fertilization rate, cleavage rate, pregnancy rate and ongoing pregnancy, but that differences did not have statistical significance.


MeSH Terms

Biological Assay
Fertilization
Fertilization in Vitro*
Iron
Metals
Pregnancy
Pregnancy Rate
Iron
Metals
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