Korean J Clin Pathol.  1999 Oct;19(5):581-586.

Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from Taegu

• Jeon CH
• Lee SC
• Sohn JH
• Ahn WS

Abstract

BACKGROUND
To investigate epidemiology of a specific strain, and evaluate correlation between Mycobacterium tuberculosis restriction fragment length polymorphism (RFLP) and antimicrobial susceptibility, we studied about Mycobacterium tuberculosis RFLP isolated from Taegu area.
METHODS
From Oct. 1997 and Mar. 1999, we isolated 54 strains of M. tuberculosis from the patients visiting Catholic University of Taegu Hyosung, Taegu, Korea. We studied their drug susceptibility and analyzed the Pvu treated RFLP using digoxigenin labeled IS6110 probe.
RESULTS
Fifty-three had more than 6 bands of RFLP and strains with 10 bands were predominant (15 strain). Cluster analysis reveals eleven distinct clusters showing 57.4% of clustered rate (31 strains from A to K) and 35 independent patterns showing 64.8% of the diversity rate at 70% similarity level. Cluster A was the largest group (7 strains) and the next was cluster B (5 strains). Most of the patients with cluster A lived in Taegu city (85.7%) and all of 2 cluster K patients lived in Euisung area. There was no correlation between RFLP pattern and antimicrobial susceptibility, but all two strains of cluster H were resistant to isoniazid. Strains of clustered were also prevalent in the people of middle class.
CONCLUSIONS
Compared to the RFLP analysis in the developed countries, Korea disclosed lower rate of diversity and higher clustered patterns of M. tuberculosis. The clustered strains were also prevalent among the people of middle class.

Keyword

Mycobacterium tuberculosis; Restriction fragment length polymorphism; Diversity rate; Taegu; Korea

MeSH Terms

Daegu*
Developed Countries
Digoxigenin
Epidemiology
Humans
Isoniazid
Korea
Mycobacterium tuberculosis*
Mycobacterium*
Polymorphism, Restriction Fragment Length*
Tuberculosis
Digoxigenin
Isoniazid