Korean J Occup Environ Med.  2000 Sep;12(3):319-326.

Mitochondrial DNA A3243G mutation in noise-induced sensorineural hearing loss

Abstract


OBJECTIVES
A different sequence change, in the mitochondrial tRNA gene, has been proposed as a candidate mutation in the sensorineurnal hearing loss. The purpose of current study is to identify the association between the noise-induced sensorineurnal hearing loss and the A to G mutation at nucleotide 3243 of mitochondrial DNA.
METHODS
Subjects were established by history and chart review, and audiological and clinical data were obtained. Blood was sampled from 101 controls, 50 noise-induced hearing loss, and 12 sensorineural deafness. The DNA of these individuals was extracted, and mitochondrial genome was analyzed by polymerase chain reaction. Subsequently, the coding sequence of mitochondrial genome was sequenced, and compared to the normal sequence, and all sequence variations were analyzed by restriction endonuclease ApaI.
RESULTS
Mitochondrial DNA mutation (3243A->G) was not detected by polymerase chain reaction (PCR) in any patients with noise-induced hearing loss, sensorineural hearing loss, and normal control without hearing loss in Koreans. The DNA sequencing of PCR products did not revealed an A to G substitution at nucleotide 3243 of mitochondrial DNA.
CONCLUSIONS
The noise-induced sensorineural hearing loss was not associated with mitochondrial DNA mutation (3243A->G)

Keyword

Mitochondrial DNA mutation; Noise-induced sensorineural hearing loss

MeSH Terms

Clinical Coding
Deafness
DNA
DNA Restriction Enzymes
DNA, Mitochondrial*
Genome, Mitochondrial
Hearing Loss
Hearing Loss, Noise-Induced
Hearing Loss, Sensorineural*
Humans
Polymerase Chain Reaction
RNA, Transfer
Sequence Analysis, DNA
DNA
DNA Restriction Enzymes
DNA, Mitochondrial
RNA, Transfer
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